Neprilysin/CD10 Antibody - BSA Free

Images

 
Western Blot: Neprilysin/CD10 Antibody [NBP2-15771] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD10 antibody [N2C1], Internal diluted at 1:500. The ...read more
Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody [NBP2-15771] - SkHep1 xenograft. CD10 antibody [N2C1], Internal dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: Neprilysin/CD10 Antibody [NBP2-15771] - A. 50 ug rat kidney lysate/extract 7.5 % SDS-PAGECD10 antibody [N2C1], Internal dilution: 1:500
Western Blot: Neprilysin/CD10 Antibody [NBP2-15771] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD10 antibody [N2C1], Internal diluted at 1:1000. The ...read more
Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody [NBP2-15771] - Mouse fore brain. CD10 antibody [N2C1], Internal dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Product Details

Summary
Reactivity Hu, Mu, Rt, RMSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free

Order Details

Neprilysin/CD10 Antibody - BSA Free Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Neprilysin/CD10. The exact sequence is proprietary.
Localization
Cell membrane; Single-pass type II membrane protein
Predicted Species
Rhesus Macaque (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MME
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence Reported in scientific literature (PMID: 30768735)
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
86 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 3
using
NBP2-15771 in the following applications:

Publications
Read Publications using
NBP2-15771 in the following applications:

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Canine (89%), Rabbit (88%), Bovine (86%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS, 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Antigen Affinity-purified

Alternate Names for Neprilysin/CD10 Antibody - BSA Free

  • Atriopeptidase
  • CALLA
  • CALLAmembrane metallo-endopeptidase (neutral endopeptidase, enkephalinase)
  • CD10 antigen
  • CD10
  • CD10)
  • CD10membrane metallo-endopeptidase variant 1
  • Common acute lymphocytic leukemia antigen
  • DKFZp686O16152
  • EC 3.4.24
  • EC 3.4.24.11
  • Enkephalinase
  • EPN
  • Leu-19
  • membrane metallo-endopeptidase variant 2
  • membrane metallo-endopeptidase
  • MGC126681
  • MGC126707
  • MME
  • NEPmembrane metallo-endopeptidase (neutral endopeptidase, enkephalinase, CALLA
  • Neprilysin
  • Neutral Endopeptidase 24.11
  • Neutral endopeptidase
  • NKH1
  • SFE
  • Skin fibroblast elastase

Background

This gene encodes a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). This protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin. This gene, which encodes a 100-kD type II transmembrane glycoprotein, exists in a single copy of greater than 45 kb. The 5' untranslated region of this gene is alternatively spliced, resulting in four separate mRNA transcripts. The coding region is not affected by alternative splicing. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Neprilysin/CD10 Antibody (NBP2-15771)(4)

Review for Neprilysin/CD10 Antibody (NBP2-15771) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-15771:
Filter by Applications
IHC-P
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin Neprilysin/CD10 NBP2-15771
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3
reviewed by:
David Krull
IHC-P Human 12/08/2016
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedhuman skin
SpeciesHuman

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WB Video Protocol
ICC/IF Video Protocol

FAQs for Neprilysin/CD10 Antibody (NBP2-15771) (0)

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David Krull
12/08/2016
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol MME
Uniprot