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Neprilysin/CD10 Antibody (7D4B1) - BSA Free

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Western Blot: Neprilysin/CD10 Antibody (7D4B1) [NBP2-37303] - Western blot analysis using CD10 mouse mAb against LNCAP cell lysate.
Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody (7D4B1) [NBP2-37303] - Analysis of paraffin-embedded cervical cancer tissues using CD10 mouse mAb with DAB staining.
Western Blot: Neprilysin/CD10 Antibody (7D4B1) [NBP2-37303] - Western blot analysis using CD10 mAb against human CD10 (AA: 52-246) recombinant protein. (Expected MW is 41.4 kDa)
Western Blot: Neprilysin/CD10 Antibody (7D4B1) [NBP2-37303] - Western blot analysis using CD10 mAb against HEK293 (1) and CD10 (AA: 52-246)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody (7D4B1) [NBP2-37303] - Analysis of paraffin-embedded prostate cancer tissues using CD10 mouse mAb with DAB staining.
ELISA: Neprilysin/CD10 Antibody (7D4B1) [NBP2-37303] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC
Clone
7D4B1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

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Catalog# & Formulation Size Price
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Neprilysin/CD10 Antibody (7D4B1) - BSA Free Summary

Immunogen
Purified recombinant fragment of human Neprilysin/CD10 (AA: 52-246) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MME
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500 - 1:2000
Theoretical MW
85.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Neprilysin/CD10 Antibody (7D4B1) - BSA Free

  • Atriopeptidase
  • CALLA
  • CALLAmembrane metallo-endopeptidase (neutral endopeptidase, enkephalinase)
  • CD10 antigen
  • CD10
  • CD10)
  • CD10membrane metallo-endopeptidase variant 1
  • Common acute lymphocytic leukemia antigen
  • DKFZp686O16152
  • EC 3.4.24
  • EC 3.4.24.11
  • Enkephalinase
  • EPN
  • Leu-19
  • membrane metallo-endopeptidase variant 2
  • membrane metallo-endopeptidase
  • MGC126681
  • MGC126707
  • MME
  • NEPmembrane metallo-endopeptidase (neutral endopeptidase, enkephalinase, CALLA
  • Neprilysin
  • Neutral Endopeptidase 24.11
  • Neutral endopeptidase
  • NKH1
  • SFE
  • Skin fibroblast elastase

Background

CD10, also known as the Common Acute Lymphocytic Leukemia Antigen (CALLA), is a cell surface enzyme with neutral metalloendopeptidase activity which inactivates a variety of biologically active peptides. CD10 is expressed on the cells of lymphoblastic, Burkitt's, and follicular germinal center lymphomas, immature B cells with in adult bone marrow and on cells from patients with chronic myelocytic leukemia (CML). CD10 is also present on breast myoepithelial cells, bile canaliculi, fibroblasts, with especially high expression on the brush border of kidney and gut epithelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol MME