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Nectin-3/PVRL3 Antibody (CL15364) [Unconjugated]

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Analysis in human cerebral cortex tissue.
Analysis in human cerebral cortex tissue.
Staining of human pancreas shows moderate membranous positivity in the islets of Langerhans.
Staining of mouse brain shows moderate positivity in neuronal processes in the CA3 area of the hippocampus.
Staining of human duodenum shows moderate to strong membranous positivity in glandular cells.
Staining of human testis shows moderate to strong membranous positivity in cells in seminiferous ducts.
Staining of human tonsil shows no positivity in non-germinal center cells as expected.
Orthogonal Strategies: Analysis in human testis and tonsil tissues using NBP3-43664 antibody. Corresponding NECTIN3 RNA-seq data are presented for the same tissues.
Staining of rat brain shows moderate positivity in neuronal processes in the CA3 area of the hippocampus.

Product Details

Summary
Reactivity Hu, Mu, Rt, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
CL15364
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml
Validated by:
       

Orthogonal Strategies

  

Order Details

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Nectin-3/PVRL3 Antibody (CL15364) [Unconjugated] Summary

Immunogen
This antibody was developed against a recombinant protein of Q9NQS3. Actual immunogen sequence is proprietary.
Predicted Species
Mouse (95%), Rat (95%). Backed by our 100% Guarantee.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NECTIN3
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:500-1:1000
  • Immunohistochemistry-Paraffin 1:500-1:1000
  • Western Blot 1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.2, 40% glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Nectin-3/PVRL3 Antibody (CL15364) [Unconjugated]

  • CD113 antigen
  • CD113
  • CDw113FLJ90624
  • DKFZp566B0846
  • nectin 3
  • Nectin3
  • Nectin-3
  • poliovirus receptor-related 3
  • poliovirus receptor-related protein 3
  • PPR3
  • PRR3CD113
  • PVRL3
  • PVRR3

Background

Nectins (e.g., PVRL1; MIM 600644) are immunoglobulin-like adhesion molecules that interact with afadin (AF6; MIM 159559). Afadin is an actin filament-binding protein that connects nectins to the actin cytoskeleton. The nectin-afadin system organizes adherens junctions cooperatively with the cadherin (see MIM 192090)-catenin (see MIM 116805) system in epithelial cells.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Nectin-3/PVRL3
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NBP3-43664
Nectin-3/PVRL3 Antibody (CL15364) [Unconjugated]
Species: Hu, Mu, Rt, Mu, Rt
Applications: WB, IHC

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Bioinformatics

Gene Symbol NECTIN3