![Immunocytochemistry/Immunofluorescence: Nectin-1/PVRL1 Antibody [NBP3-12960] - Nectin-1/PVRL1 antibody detects Nectin-1/PVRL1 protein by immunofluorescent analysis. Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: Nectin-1/PVRL1 protein stained by Nectin-1/PVRL1 antibody (NBP3-12960) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (NBP3-13505) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Nectin-1-PVRL1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-12960-img0002.jpg "Immunocytochemistry/Immunofluorescence: Nectin-1/PVRL1 Antibody [NBP3-12960] - Nectin-1/PVRL1 antibody detects Nectin-1/PVRL1 protein by immunofluorescent analysis. Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: Nectin-1/PVRL1 protein stained by Nectin-1/PVRL1 antibody (NBP3-12960) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (NBP3-13505) diluted at 1:500. Blue: Fluoroshield with DAPI.")
| Reactivity | Hu, Mu, Rt, Po, CaSpecies Glossary |
| Applications | WB, ICC/IF |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | Centrifuge briefly prior to opening. |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human Nectin-1/PVRL1. The exact sequence is proprietary. |
| Predicted Species | Porcine (96%), Canine (95%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | NECTIN1 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Nectin-1/PVRL1 Antibody (NBP3-12960)Find related products by research area.
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| Gene Symbol | NECTIN1 |
![Western Blot: Nectin-1/PVRL1 Antibody [NBP3-12960] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Nectin-1/PVRL1 antibody (NBP3-12960) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-12960_rabbit-nectin-1-pvrl1-pab-91020242001092.jpg "Western Blot: Nectin-1/PVRL1 Antibody [NBP3-12960] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Nectin-1/PVRL1 antibody (NBP3-12960) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Nectin-1/PVRL1 Antibody [NBP3-12960] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Nectin 1 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-12960_rabbit-nectin-1-pvrl1-pab-1610202419405730.jpg "Western Blot: Nectin-1/PVRL1 Antibody [NBP3-12960] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Nectin 1 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
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