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Ndufs4 Antibody (6T10F4)

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Western Blot: Ndufs4 Antibody (6T10F4) [NBP3-16679] - Western blot analysis of extracts of various cell lines, using Ndufs4 Rabbit mAb (NBP3-16679) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) ...read more
Immunocytochemistry/ Immunofluorescence: Ndufs4 Antibody (6T10F4) [NBP3-16679] - Immunofluorescence analysis of NIH-3T3 cells using Ndufs4 Rabbit mAb (NBP3-16679) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear ...read more
Western Blot: Ndufs4 Antibody (6T10F4) [NBP3-16679] - Western blot analysis of extracts of HepG2 cells, using Ndufs4 Rabbit mAb (NBP3-16679) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at ...read more
Immunocytochemistry/ Immunofluorescence: Ndufs4 Antibody (6T10F4) [NBP3-16679] - Immunofluorescence analysis of C6 cells using Ndufs4 Rabbit mAb (NBP3-16679) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear ...read more
Immunohistochemistry-Paraffin: Ndufs4 Antibody (6T10F4) [NBP3-16679] - Analysis of NDUFS4 in paraffin-embedded human colon carcinoma tissue using NDUFS4 Rabbit mAb at a dilution of 1:400 (40x lens). High pressure ...read more
Immunohistochemistry-Paraffin: Ndufs4 Antibody (6T10F4) [NBP3-16679] - Analysis of NDUFS4 in paraffin-embedded human liver tissue using NDUFS4 Rabbit mAb at a dilution of 1:400 (40x lens). High pressure antigen ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
6T10F4
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Ndufs4 Antibody (6T10F4) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human NDUFS4 (O43181). MAAVSMSVVLRQTLWRRRAVAVAALSVSRVPTRSLRTSTWRLAQDQTQDTQLITVDEKLDITTLTGVPEEHIKTRKVRIFVPARNNMQSGVNNTKKWKME
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
NDUFS4
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for Ndufs4 Antibody (6T10F4)

  • AQDQ
  • AQDQmitochondrial
  • CI-18 kDa
  • NADH dehydrogenase (ubiquinone) Fe-S protein 4 (18kD) (NADH-coenzyme Qreductase)
  • NADH dehydrogenase (ubiquinone) Fe-S protein 4, 18kDa (NADH-coenzyme Qreductase)
  • NADH-coenzyme Q reductase, 18-KD
  • NADH-ubiquinone oxidoreductase 18 kDa subunit

Background

Ndufs4 encodes an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), or NADH:ubiquinone oxidoreductase, the first multi-subunit enzyme complex of the mitochondrial respiratory chain. Complex I plays a vital role in cellular ATP production, the primary source of energy for many crucial processes in living cells. It removes electrons from NADH and passes them by a series of different protein-coupled redox centers to the electron acceptor ubiquinone. In well-coupled mitochondria, the electron flux leads to ATP generation via the building of a proton gradient across the inner membrane. Complex I is composed of at least 41 subunits, of which 7 are encoded by the mitochondrial genome and the remainder by nuclear genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NDUFS4