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Ndufs1 Antibody

Images

 
Western Blot: Ndufs1 Antibody [NBP1-31142] - Effect of BGP-15 treatment on the electron transport chain complex I and III proteins in NRCMs. Western blot analysis of UQCRC1 (NBP2-03825) and Ndufs1 (NBP1-31142) proteins ...read more
Immunohistochemistry-Paraffin: Ndufs1 Antibody [NBP1-31142] - Ovarian carcinoma. NDUFS1 antibody dilution: 1:250. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Western Blot: Ndufs1 Antibody [NBP1-31142] - 50 ug mouse brain lysate/extract 7. 5 % SDS-PAGE gel, antibody dilution 1:500.
Western Blot: Ndufs1 Antibody [NBP1-31142] - Hep G2 7.5% SDS PAGE diluted at 1:1500
Western Blot: Ndufs1 Antibody [NBP1-31142] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE; antibody diluted at 1:10000.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, PmSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Ndufs1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Ndufs1. The exact sequence is proprietary.
Localization
Mitochondrion inner membrane
Predicted Species
Bovine (96%), Chimpanzee (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NDUFS1
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:10000
Theoretical MW
79 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP1-31142 in the following applications:

  • WB
    2 publications

Reactivity Notes

Xenopus laevis (82%), Chicken (86%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for Ndufs1 Antibody

  • CI-75k
  • CI-75kD
  • complex I 75kDa subunit
  • complex I, mitochondrial respiratory chain, 75-kD subunit
  • Complex I-75kD
  • EC 1.6.5.3
  • EC 1.6.99.3
  • EC 7.1.1.2
  • MGC26839
  • mitochondrial NADH-ubiquinone oxidoreductase 75 kDa subunit
  • NADH dehydrogenase (ubiquinone) Fe-S protein 1 (75kD) (NADH-coenzyme Qreductase)
  • NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa (NADH-coenzyme Qreductase)
  • NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial
  • Ndufs1
  • PRO1304

Background

The protein encoded by this gene belongs to the complex I 75 kDa subunit family. Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. This protein is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized. Mutations in this gene are associated with complex I deficiency. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Ndufs1 Antibody (NBP1-31142)(2)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NDUFS1