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NDUFAF2 Antibody

Images

 
Independent Antibodies: Western Blot: NDUFAF2 Antibody [NBP2-47359] - Analysis using Anti-NDUFAF2 antibody NBP2-47359 (A) shows similar pattern to independent antibody NBP2-56048 (B).
Immunocytochemistry/ Immunofluorescence: NDUFAF2 Antibody [NBP2-47359] - Immunofluorescent staining of human cell line HEK 293 shows localization to mitochondria.
Immunohistochemistry: NDUFAF2 Antibody [NBP2-47359] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
 

Independent Antibodies

       

Order Details

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NDUFAF2 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: MEEILKNEKHREEIKIKSQDFYEKEKLLSKETSEELLPPPVQTQIKGHASAPYFGKEEPSVAPSSTGKTFQPGSWMPRDGKSHNQ
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NDUFAF2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
NDUFAF2 Protein (NBP2-47359PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (82%), Rat (81%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for NDUFAF2 Antibody

  • B17.2LFLJ22398
  • B17.2-like
  • mimitin
  • MMTNmitochondrial
  • Myc-induced mitochondrial protein
  • NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 2
  • NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 2
  • NDUFA12-like protein
  • NDUFA12-like

Background

NADH:ubiquinone oxidoreductase (complex I) catalyzes the transfer of electrons from NADH to ubiquinone (coenzyme Q) inthe first step of the mitochondrial respiratory chain, resulting in the translocation of protons across the innermitochondrial membrane. This gene encodes a complex I assembly factor. Mutations in this gene cause progressiveencephalopathy resulting from mitochondrial complex I deficiency. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NDUFAF2 Antibody (NBP2-47359) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NDUFAF2