NDC80 Antibody

Images

 
Western Blot: NDC80 Antibody [NB100-1100] - Staining (0.5 ug/ml) of Hela lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: NDC80 Antibody [NB100-1100] - Staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: NDC80 Antibody [NB100-1100] - Staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

NDC80 Antibody Summary

Immunogen
Peptide with sequence C-YEKKATLIKSSEE corresponding to C-Terminus according to NP_006092.1.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
NDC80
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.5 - 2 ug/ml
Application Notes
WB: Approx. 75-80 kDa band observed in Hela lysate (predicted MW of 74 kDa band according to NP_006092). IHC: Paraffin embedded Human Liver and Adrenal Gland.
Control
HeLa Whole Cell Lysate
Publications
Read Publications using NB100-1100.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NDC80 Antibody

  • HEC1KNTC2
  • HECRetinoblastoma-associated protein HEC
  • Highly expressed in cancer protein
  • highly expressed in cancer, rich in leucine heptad repeats (yeast)
  • highly expressed in cancer, rich in leucine heptad repeats
  • hsHec1
  • hsNDC80
  • kinetochore associated 2
  • Kinetochore protein Hec1
  • kinetochore protein NDC80 homolog
  • Kinetochore-associated protein 2
  • NDC80 homolog, kinetochore complex component (S. cerevisiae)
  • TID3

Background

HEC is one of several proteins involved in spindle checkpoint signaling. This surveillance mechanism assures correct segregation of chromosomes during cell division by detecting unaligned chromosomes and causing prometaphase arrest until the proper bipolar attachment of chromosomes is achieved.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-31632
Species: Hu
Applications: ICC/IF, WB
NBP2-47264
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP3-13389
Species: Hu
Applications: IHC, IHC-P, WB
NB100-563
Species: Hu
Applications: ICC/IF, IHC, IHC-P, IP, WB
NB100-2359
Species: Hu
Applications: IHC, IP, WB
NBP2-47263
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
AF4185
Species: Hu
Applications: ICC
NB100-294
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, MiAr, WB
NBP1-32775
Species: Hu, Mar, Mu, Rt
Applications: ChIP, ICC/IF, IHC, IHC-P, IP, WB
NBP2-85249
Species: Hu, Mu
Applications: WB
NB100-2586
Species: Hu
Applications: ICC/IF, IP, WB
NB110-40681
Species: Hu, Mu
Applications: IHC, IHC-P, IP, WB
NBP1-82546
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
AF6028
Species: Hu
Applications: IHC, WB
M6000B
Species: Mu
Applications: ELISA
AF1730
Species: Hu
Applications: AdBlk, CyTOF-ready, Flow, ICC
NB100-1100
Species: Hu
Applications: WB, ELISA, IHC

Publications for NDC80 Antibody (NB100-1100)(2)

Reviews for NDC80 Antibody (NB100-1100) (0)

There are no reviews for NDC80 Antibody (NB100-1100). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for NDC80 Antibody (NB100-1100) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional NDC80 Products

Array NB100-1100

Research Areas for NDC80 Antibody (NB100-1100)

Find related products by research area.

Blogs on NDC80

There are no specific blogs for NDC80, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our NDC80 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol NDC80
Entrez
Uniprot