![Western Blot: Myosin light chain kinase Antibody [H00004638-D01P] - Analysis of MYLK expression in human stomach.](http://images.novusbio.com/fullsize/Myosin-light-chain-kinase-Antibody-Western-Blot-H00004638-D01P-img0004.jpg "Western Blot: Myosin light chain kinase Antibody [H00004638-D01P] - Analysis of MYLK expression in human stomach.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, Func |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | Quality control test: Antibody reactive against mammalian transfected lysate. |
| Immunogen | MYLK (ENSP00000355024, 1 a.a. - 992 a.a.) full-length human protein. MDFRANLQRQVKPKTVSEEERKVHSPQQVDFRSVLAKKGTSKTPVPEKVPPPKPATPDFRSVLGGKKKLPAENGSSSAETLNAKAVESSKPLSNAQPSGPLKPVGNAKPAETLKPMGNAKPAETLKPMGNAKPDENLKSASKEELKKDVKNDVNCKRGHAGTTDNEKRSESQGTAPAFKQKLQDVHVAEGKKLLLQCQVSSDPPATIIWTLNGKTLKTTKFIILSQEGSLCSVSIEKALPEDRGLYKCVAKNDAGQAECSCQVTVDDAPASENTKAPEMKSRRPKSSLPPVLGTESDATVKKKPAPKTPPKAAMPPQIIQFPEDQKVRAGESVELFGKVTGTQPITCTWMKFRKQIQESEHMKVENSENGSKLTILAARQEHCGCYTLLVENKLGSRQAQVNLTVVDKPDPPAGTPCASDIRSSSLTLSWYGSSYDGGSAVQSYSIEIWDSANKTWKELATCRSTSFNVQDLLPDHEYKFRVRAINVYGTSEPSQESELTTVGEKPEEPKDEVEVSDDDEKEPEVDYRTVTINTEQKVSDFYDIEERLGSGKFGQVFRLVEKKTRKVWAGKFFKAYSAKEKENIRQEISIMNCLHHPKLVQCVDAFEEKANIVMVLEIVSGGELFERIIDEDFELTERECIKYMRQISEGVEYIHKQGIVHLDLKPENIMCVNKTGTRIKLIDFGLARRLENAGSLKVLFGTPEFVAPEVINYEPIGYATDMWSIGVICYILVSGLSPFMGDNDNETLANVTSATWDFDDEAFDEISDDAKDFISNLLKKDMKNRLDCTQCLQHPWLMKDTKNMEAKKLSKDRMKKYMARRKWQKTGNAVRAIGRLSSMAMISGLSGRKSSTGSPTSPLNAEKLESEEDVSQAFLEAVAEEKPHVKPYFSKTIRDLEVVEGSAARFDCKIEGYPDPEVVWFKDDQSIRESRHFQIDYDEDGNCSLIISDVCGDDDAKYTCKAVNSLGEATCTAELIVETMEEGEGEGEEEEE |
| Specificity | MYLK - myosin, light chain kinase, |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | MYLK |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.4) |
| Preservative | No Preservative |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Myosin light chain kinase Antibody (H00004638-D01P)Find related products by research area.
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| Gene Symbol | MYLK |
| Entrez |
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| Uniprot |
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![Western Blot: Myosin light chain kinase Antibody [H00004638-D01P] - Analysis of MYLK expression in transfected 293T cell line by MYLK polyclonal antibody.Lane 1: MYLK transfected lysate(110.20 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Myosin-light-chain-kinase-Antibody-Western-Blot-H00004638-D01P-img0005.jpg "Western Blot: Myosin light chain kinase Antibody [H00004638-D01P] - Analysis of MYLK expression in transfected 293T cell line by MYLK polyclonal antibody.Lane 1: MYLK transfected lysate(110.20 KDa).Lane 2: Non-transfected lysate.")
![Proximity Ligation Assay: Myosin light chain kinase Antibody [H00004638-D01P] - Analysis of protein-protein interactions between MYLK and CTTN. HeLa cells were stained with anti-MYLK rabbit purified polyclonal 1:1200 and anti-CTTN mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Myosin-light-chain-kinase-Antibody-Proximity-Ligation-Assay-H00004638-D01P-img0003.jpg "Proximity Ligation Assay: Myosin light chain kinase Antibody [H00004638-D01P] - Analysis of protein-protein interactions between MYLK and CTTN. HeLa cells were stained with anti-MYLK rabbit purified polyclonal 1:1200 and anti-CTTN mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).")
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![Immunohistochemistry MRCL3 [p Ser19, p Ser20] Antibody](https://images.novusbio.com/images/nb100-1687_rabbit-polyclonal-mrcl3-p-ser19-p-ser20-antibody-23520231814583.jpg)
![Immunohistochemistry MRCL3 [p Ser19, p Ser20] Antibody](https://images.novusbio.com/images/nb100-1687_rabbit-polyclonal-mrcl3-p-ser19-p-ser20-antibody-235202318143193.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")