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Myosin light chain kinase Antibody (1J2U2)

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Western Blot: Myosin light chain kinase Antibody (1J2U2) [NBP3-16282] - Western blot analysis of extracts of various cell lines, using Myosin light chain kinase Rabbit mAb (NBP3-16282) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Western Blot: Myosin light chain kinase Antibody (1J2U2) [NBP3-16282] - Western blot analysis of extracts of various cell lines, using Myosin light chain kinase Rabbit mAb (NBP3-16282) at 1:1000 dilution. Secondary ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB
Clone
1J2U2
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Myosin light chain kinase Antibody (1J2U2) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1800-1900 of human Myosin light chain kinase (Q15746). VAEEKPHVKPYFSKTIRDLEVVEGSAARFDCKIEGYPDPEVVWFKDDQSIRESRHFQIDYDEDGNCSLIISDVCGDDDAKYTCKAVNSLGEATCTAELIVE
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
MYLK
Purity
Affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:2000

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for Myosin light chain kinase Antibody (1J2U2)

  • DKFZp686I10125
  • EC 2.7.11
  • EC 2.7.11.18
  • FLJ12216
  • Kinase-related protein
  • KRP
  • light polypeptide kinase
  • MLCK1
  • MLCK108
  • MLCKMLCK210
  • MSTP083
  • MYLK1
  • myosin light chain kinase
  • smooth muscle myosin light chain kinase
  • smooth muscle
  • telokin

Background

Myosin light chain kinase (MLCK) is a serine/threonine kinase that belongs to CaM Kinases (Ca++/Calmodulin-dependent protein kinases) family. MLCK is involved in the regulation of myosin activity during smooth muscle contraction. At increased level of Ca2+, rapid binding of Ca2+ to Calmodulin (CaM) occurs, triggering MLCK activation. Once activated, MLCK phosphorylates myosin light chain (MLC) and leading to cross-bridges cycling and generation of the contractile force (1). MLCK has been implicated in the regulation of endothelial and vascular permeability and in signaling pathway which leads to fibroblast apoptosis (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Myosin light chain
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NBP3-16282
Myosin light chain kinase Antibody (1J2U2)
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Bioinformatics

Gene Symbol MYLK