![Western Blot: Myelin expression factor 2 Antibody [NBP3-12682] - Lane A: SH-SY5Y Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:64 kDaObserved band size:70 kDa](http://images.novusbio.com/fullsize/Myelin-expression-factor-2-Antibody-Western-Blot-NBP3-12682-img0001.jpg "Western Blot: Myelin expression factor 2 Antibody [NBP3-12682] - Lane A: SH-SY5Y Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:64 kDaObserved band size:70 kDa")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles. Protein A & Antigen Affinity purified |
| Immunogen | Produced in rabbits immunized with E. coli-derived Human Myelin expression factor 2 fragment |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | MyEF2 |
| Purity | Antigen and protein A Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.03% Proclin 300 |
| Purity | Antigen and protein A Affinity-purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | MyEF2 |
![Immunocytochemistry/Immunofluorescence: Myelin expression factor 2 Antibody [NBP3-12682] - Staining of MYEF2 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human MYEF2 polyclonal antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/Myelin-expression-factor-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-12682-img0005.jpg "Immunocytochemistry/Immunofluorescence: Myelin expression factor 2 Antibody [NBP3-12682] - Staining of MYEF2 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human MYEF2 polyclonal antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.")
![Immunohistochemistry-Paraffin: Myelin expression factor 2 Antibody [NBP3-12682] - Staining of human MYEF2 in human kidney with rabbit polyclonal antibody at 1:10000 dilution.](http://images.novusbio.com/fullsize/Myelin-expression-factor-2-Antibody-Immunohistochemistry-Paraffin-NBP3-12682-img0004.jpg "Immunohistochemistry-Paraffin: Myelin expression factor 2 Antibody [NBP3-12682] - Staining of human MYEF2 in human kidney with rabbit polyclonal antibody at 1:10000 dilution.")
![Immunohistochemistry-Paraffin: Myelin expression factor 2 Antibody [NBP3-12682] - Staining of human MYEF2 in human testis with rabbit polyclonal antibody at 1:10000 dilution.](http://images.novusbio.com/fullsize/Myelin-expression-factor-2-Antibody-Immunohistochemistry-Paraffin-NBP3-12682-img0002.jpg "Immunohistochemistry-Paraffin: Myelin expression factor 2 Antibody [NBP3-12682] - Staining of human MYEF2 in human testis with rabbit polyclonal antibody at 1:10000 dilution.")
![Immunohistochemistry-Paraffin: Myelin expression factor 2 Antibody [NBP3-12682] - Staining of human MYEF2 in human brain with rabbit polyclonal antibody at 1:10000 dilution.](http://images.novusbio.com/fullsize/Myelin-expression-factor-2-Antibody-Immunohistochemistry-Paraffin-NBP3-12682-img0003.jpg "Immunohistochemistry-Paraffin: Myelin expression factor 2 Antibody [NBP3-12682] - Staining of human MYEF2 in human brain with rabbit polyclonal antibody at 1:10000 dilution.")
![Immunoprecipitation: Myelin expression factor 2 Antibody [NBP3-12682] - Lane A:0.5 mg SH-SY5Y Whole Cell Lysate4 uL anti-MYEF2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-MYEF2 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 64 kDaObserved band size :64 kDa](http://images.novusbio.com/fullsize/Myelin-expression-factor-2-Antibody-Immunoprecipitation-NBP3-12682-img0006.jpg "Immunoprecipitation: Myelin expression factor 2 Antibody [NBP3-12682] - Lane A:0.5 mg SH-SY5Y Whole Cell Lysate4 uL anti-MYEF2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-MYEF2 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 64 kDaObserved band size :64 kDa")
![N/A PSG1 [HRP]](https://images.novusbio.com/images2/DATA_PSG1_DPSG10_ELISA_797.jpg)
![N/A PSG1 [HRP]](https://images.novusbio.com/images2/PSG-1_DPSG10_ELISA_164.jpg)
![Western Blot NKX2.5 Antibody [Unconjugated]](https://images.novusbio.com/images/af2444_human-nkx2-5-affinity-purified-polyclonal-ab-4120241239327.jpg)
![Immunocytochemistry/ Immunofluorescence NKX2.5 Antibody [Unconjugated]](https://images.novusbio.com/images/af2444_human-nkx2-5-affinity-purified-polyclonal-ab-41202412401270.jpg)
![Immunocytochemistry/ Immunofluorescence NKX2.5 Antibody [Unconjugated]](https://images.novusbio.com/images/af2444_human-nkx2-5-affinity-purified-polyclonal-ab-41202412403531.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")