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Human Muscarinic Acetylcholine Receptor M1/CHRM1 ELISA Kit (Colorimetric)

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ELISA: Human Muscarinic Acetylcholine Receptor M1/CHRM1 ELISA Kit (Colorimetric) [NBP2-81154] - Samples were spiked with high concentrations of Human Muscarinic Acetylcholine Receptor M1/CHRM1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Human Muscarinic Acetylcholine Receptor M1/CHRM1 ELISA Kit (Colorimetric) [NBP2-81154] - Samples were spiked with high concentrations of Human Muscarinic Acetylcholine Receptor M1/CHRM1 and diluted with Reference ...read more
ELISA: Human Muscarinic Acetylcholine Receptor M1/CHRM1 ELISA Kit (Colorimetric) [NBP2-81154] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, cell supernatant, and other biological fluids
Standard Curve Range
0.31 - 20 ng/mL
Sensitivity
0.19 ng/mL

Order Details

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Human Muscarinic Acetylcholine Receptor M1/CHRM1 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human Muscarinic Acetylcholine Receptor M1/CHRM1 in samples. No significant cross-reactivity or interference between Human Muscarinic Acetylcholine Receptor M1/CHRM1 and analogues was observed.
Standard Curve Range
0.31 - 20 ng/mL
Sensitivity
0.19 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5.49%
Intra-Assay
CV% < 5.11%
Spike Recovery
86-105%
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
CHRM1

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human Muscarinic Acetylcholine Receptor M1/CHRM1 ELISA Kit (Colorimetric)

  • cholinergic receptor, muscarinic 1
  • CHRM1
  • HM1
  • M1
  • M1R
  • mAChR M1
  • MGC30125
  • muscarinic acetylcholine receptor M1

Background

The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 1 is involved in mediation of vagally-induced bronchoconstriction and in the acid secretion of the gastrointestinal tract. The gene encoding this receptor is localized to 11q13.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product PDFs

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  Human Muscarinic Acetylcholine Receptor M1/CHRM1 ELISA Kit (Colorimetric) Manual
  PDF Datasheet
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Bioinformatics

Gene Symbol CHRM1