Reactivity | Hu, Mu, Rt, Po, Ch, Fe, Ma, Pm, Rb, Bv(-)Species Glossary |
Applications | WB, DB, Flow, ICC/IF, IHC, CyTOF-ready |
Clone | 45M1 |
Clonality | Monoclonal |
Host | Mouse |
Conjugate | Unconjugated |
Format | Azide and BSA Free |
Concentration | 1.0 mg/ml |
Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-15196). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
Immunogen | M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient |
Localization | Cytoplasmic |
Specificity | This monoclonal antibody recognizes the peptide core of gastric mucin M1 (recently identified as Mucin 5AC).Its epitope is located in the C-terminal cysteine rich part of the peptide core of MUC5AC. Its epitope is destroyed by beta-mercaptoethanol but not by periodate treatment. This mucin is present in primary ovarian mucinous cancer but usually absent in colorectal adenocarcinoma, thus showing an expression pattern opposite to MUC2. Together with a panel of antibodies, Anti-MUC5AC may be useful for differential identification of primary mucinous ovarian tumors from colon adenocarcinoma metastatic to the ovary. MUC5AC antibodies may also be useful for identification of intestinal metaplasia as well as in the identification of pancreatic carcinoma and pre-cancerous changes vs. normal pancreas. |
Isotype | IgG1 Kappa |
Clonality | Monoclonal |
Host | Mouse |
Gene | MUC5AC |
Purity | Protein A or G purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | Use in Dot Blot reported in scientific literature (PMID: 31374872). Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
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Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
Buffer | 10 mM PBS |
Preservative | No Preservative |
Concentration | 1.0 mg/ml |
Purity | Protein A or G purified |
Publications using NBP2-32732 | Applications | Species |
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Gindele Ja Characterization of the molecular mechanisms underlying the pathophysiological differentiation of small airway epithelial cells in Chronic Obstructive Pulmonary Disease (COPD) Thesis 2021-01-01 | ||
Castaneda DC, Jangra S, Yurieva M et al. Spatiotemporally organized immunomodulatory response to SARS-CoV-2 virus in primary human broncho-alveolar epithelia bioRxiv : the preprint server for biology 2023-03-31 [PMID: 37034597] (ICC/IF, Human) | ICC/IF | Human |
Koh K, Bonser L, Eckalbar W et al. Genomic characterization and therapeutic utilization of IL-13-responsive sequences in asthma Cell Genomics 2022-12-01 [PMID: 36777184] (FLOW, Human) | FLOW | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for MUC5AC Antibody (NBP2-32732)Find related products by research area.
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Gene Symbol | MUC5AC |