MUC2 Antibody (CCP58)

MUC2, also called mucin 2, is a member of the mucin protein family which are heavily glycosylated, high molecular weight proteins that are produced by goblet cells in epithelial tissues including the intestinal and respiratory tract (1). Mucins can form gels and function in lubrication of mucosal surfaces which serve as a protective barrier of the epithelial lining and an innate host defense (1). Mucins are divided into two groups based on whether they are membrane-bound or secreted, where MUC2 belongs to the secretory group (1,2). Secreted mucins have a distinctive composition characterized by a large central exon "mucin domain" containing O-glycosylated tandem repeat regions rich in proline, threonine, and serine residues, non-repetitive domains, and cysteine-rich domains (1,2). The mucin domain is flanked by 5' and 3' D domains, a B-like domain, a C-like domain, and a Ck-domain (1,2). MUC2 was the first secretory mucin identified in humans (3,4). Full-length MUC2 protein is synthesized as 5179 amino acids (aa) in length with a theoretical molecular weight of ~540 kDa (5).

Changes or perturbations to MUC2 expression has been associated with a number of disease pathologies (1-4, 6). Specifically, altered MUC2 composition has been indicated in colorectal cancer, inflammatory bowel diseases (IBD) including ulcerative colitis and Chron's disease, and chronic obstructive pulmonary disease (COPD) (1-4, 6). In general, decreased or reduced MUC2 expression is associated with colorectal cancer disease progression and development of IBD (1-4, 6). Additionally, studies have found that upon intestinal infection due to parasites or bacteria MUC2 expression is increased, as is overall mucus secretion (3, 6).

References

1. Pothuraju, R., Krishn, S. R., Gautam, S. K., Pai, P., Ganguly, K., Chaudhary, S., Rachagani, S., Kaur, S., & Batra, S. K. (2020). Mechanistic and Functional Shades of Mucins and Associated Glycans in Colon Cancer. Cancers. https://doi.org/10.3390/cancers12030649

2. Ballester, B., Milara, J., & Cortijo, J. (2019). Mucins as a New Frontier in Pulmonary Fibrosis. Journal of Clinical Medicine. https://doi.org/10.3390/jcm8091447

3. Liu, Y., Yu, X., Zhao, J., Zhang, H., Zhai, Q., & Chen, W. (2020). The role of MUC2 mucin in intestinal homeostasis and the impact of dietary components on MUC2 expression. International Journal of Biological Macromolecules. https://doi.org/10.1016/j.ijbiomac.2020.07.191

4. Allen, A., Hutton, D. A., & Pearson, J. P. (1998). The MUC2 gene product: a human intestinal mucin. The International Journal of Biochemistry & Cell Biology. https://doi.org/10.1016/s1357-2725(98)00028-4

5. Uniprot (Q02817)

6. Kim, Y. S., & Ho, S. B. (2010). Intestinal goblet cells and mucins in health and disease: recent insights and progress. Current Gastroenterology Reports. https://doi.org/10.1007/s11894-010-0131-2

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: IF/IHC, IHC-P, IP, WB.

Showing Publications 1 - 7 of 7.

Publications using NBP2-25221	Applications	Species
Wu D, Su S, Zha X et al. Glutamine promotes O-GlcNAcylation of G6PD and inhibits AGR2 S-glutathionylation to maintain the intestinal mucus barrier in burned septic mice Redox biology 2022-12-20 [PMID: 36565645] (WB, Mouse, Human)	WB	Mouse, Human
Al-Shaibi AA, Abdel-Motal UM, Hubrack SZ et al. Human AGR2 Deficiency Causes Mucus Barrier Dysfunction and Infantile Inflammatory Bowel Disease Cellular and Molecular Gastroenterology and Hepatology 2021-07-06 [PMID: 34237462] (WB, IP)	WB, IP
Kobayashi T, Omori Y, Ono Y et al. Pathways for the development of multiple epithelial types of intraductal papillary mucinous neoplasm of the pancreas Journal of gastroenterology 2021-04-01 [PMID: 33796937]
Hermelijn SM, Wolf JL, Dorine den Toom T et al. Early KRAS oncogenic driver mutations in non-mucinous tissue of Congenital Pulmonary Airway Malformations as an indicator of potential malignant behavior Hum. Pathol. 2020-07-15 [PMID: 32681943] (IHC-P, Human)	IHC-P	Human
Aoki Y, Mizuma M, Hata T et al. Intraductal papillary neoplasms of the bile duct are consisted of two distinct types specifically associated with clinicopathological features and molecular phenotypes J Pathol. [PMID: 32100878] (IF/IHC, Human)	IF/IHC	Human
Workman MJ, Gleeson JP, Troisi EJ et al. Enhanced Utilization of Induced Pluripotent Stem Cell-Derived Human Intestinal Organoids Using Microengineered Chips Cell Mol Gastroenterol Hepatol 2017-12-29 [PMID: 29930984] (Human)		Human
Salerno-Goncalves R, Safavie F, Fasano A, Sztein MB. Free and complexed-secretory immunoglobulin A triggers distinct intestinal epithelial responses. Clin. Exp. Immunol. 2016-04-16 [PMID: 27084834] (IHC-P, WB, Human)	IHC-P, WB	Human

Images	Ratings	Applications	Species	Date	Details
Enlarge	4 reviewed by: Verified Customer	IHC-P	Human	04/04/2020	View Summary Application Immunohistochemistry-Paraffin Sample Tested Breast cancer tissue Species Human Lot 4583-1P170511
Enlarge	4 reviewed by: Verified Customer	WB	Human	12/27/2019	View Summary Application Western Blot Sample Tested MDA MB 231 cells Species Human Lot 4583-1p170511
Enlarge	5 reviewed by: Verified Customer	WB	Human	10/17/2018	View Summary Application Western Blot Sample Tested HCC827 human non-small cell lung cancer cell line,MDA MB 231 cells Species Human Lot 4583-1P170511

Array NBP2-25221

• MUC2 Antibodies
• MUC2 ELISA Kits