mtRNA polymerase Antibody

Images

 
Western Blot: mtRNA polymerase Antibody [NBP2-19436] - Sample (30 ug of whole cell lysate) A: Hep G2 5% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: mtRNA polymerase Antibody [NBP2-19436] - Immunofluorescence analysis of methanol-fixed Hep G2, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: mtRNA polymerase Antibody [NBP2-19436] - Human hepatoma, using mtRNA polymerase antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

mtRNA polymerase Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human mtRNA polymerase. The exact sequence is proprietary.
Localization
Mitochondrion
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
POLRMT
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
139 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS, 1% BSA, 20% Glycerol (pH7)
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for mtRNA polymerase Antibody

  • APOLMTEC 2.7.7.6
  • DNA-directed RNA polymerase, mitochondrial
  • EC 2.7.7.6
  • h-mtRPOL
  • mtRNA polymerase
  • MTRNAP
  • MtRPOL
  • POLRMT
  • polymerase (RNA) mitochondrial (DNA directed)

Background

This gene encodes a mitochondrial DNA-directed RNA polymerase. The gene product is responsible for mitochondrial gene expression as well as for providing RNA primers for initiation of replication of the mitochondrial genome. Although this polypeptide has the same function as the three nuclear DNA-directed RNA polymerases, it is more closely related to RNA polymerases of phage and mitochondrial polymerases of lower eukaryotes. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol POLRMT
Uniprot