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MTHFR Antibody

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Western Blot: MTHFR Antibody [NBP2-27549] - (0.3 ug/ml) staining of NIH3T3 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity Mu, Hu, Rt, Po, Bv, CaSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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MTHFR Antibody Summary

Immunogen
Peptide with sequence C-SRPKSYIYRTQEWDE corresponding to internal region according to NP_005948.3.
Predicted Species
Human (100%), Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MTHFR
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 0.3 - 1 ug/ml
Application Notes
WB: Approx. 70 kDa band observed in NIH3T3 lysates (calculated MW of 74.6 kDa band according to human NP_005948.3 and mouse NP_034970.2).
Publications
Read Publication using NBP2-27549.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MTHFR Antibody

  • EC 1.5.1.20
  • methylenetetrahydrofolate reductase (NAD(P)H)5,10-methylenetetrahydrofolate reductase (NADPH)
  • methylenetetrahydrofolate reductase

Background

Methylenetetrahydrofolate reductase (EC 1.5.1.20) catalyzes the conversion of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, a cosubstrate for homocysteine remethylation to methionine.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MTHFR Antibody (NBP2-27549)(1)


Showing Publication 1 - 1 of 1.
Publication using NBP2-27549 Applications Species
Fletcher O, Kessling AM. MTHFR association with arteriosclerotic vascular disease? Hum Genet 1998-07-01 [PMID: 9737770]

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Product General Protocols

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Video Protocols

WB Video Protocol

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Bioinformatics

Gene Symbol MTHFR
Entrez
Uniprot