![Immunohistochemistry-Paraffin: MTHFR Antibody [NBP1-45222] - (3.75ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/MTHFR-Antibody-Immunohistochemistry-Paraffin-NBP1-45222-img0001.jpg "Immunohistochemistry-Paraffin: MTHFR Antibody [NBP1-45222] - (3.75ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, IHC |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence C-NRPTQNARETEAP corresponding to C-Terminus according to NP_005948.3. |
| Epitope | C-Terminus |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | MTHFR |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Western blot: Preliminary experiments gave an approx 28kDa band in Human Brain (Cerebellum), Skeletal Muscle and Placenta lysates after 0.2ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 74.6kDa according to NP_005948.3. The 28kDa band was successfully blocked by incubation with the immunizing peptide. IHC: Paraffin embedded Human Spleen. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
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| Publication using NBP1-45222 | Applications | Species |
|---|---|---|
| Bethke L, Webb E, Murray A et al. Functional polymorphisms in folate metabolism genes influence the risk of meningioma and glioma. Cancer Epidemiol Biomarkers Prev 2008-05-01 [PMID: 18483342] |
Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | MTHFR |
| Entrez |
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| Uniprot |
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