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MPP3 Antibody

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Western Blot: MPP3 Antibody [NBP1-85014] - Analysis in control (vector only transfected HEK293T lysate) and MPP3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: MPP3 Antibody [NBP1-85014] - Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
Immunohistochemistry-Paraffin: MPP3 Antibody [NBP1-85014] - Staining of human liver shows no in hepatocytes as expected.
Immunohistochemistry-Paraffin: MPP3 Antibody [NBP1-85014] - Staining of human stomach shows strong cytoplasmic positivity in parietal cells.
Immunohistochemistry-Paraffin: MPP3 Antibody [NBP1-85014] - Staining of human cerebellum shows moderate positivity in neuropil.
Immunohistochemistry-Paraffin: MPP3 Antibody [NBP1-85014] - Staining of human kidney shows strong cytoplasmic positivity in cells in distal tubules.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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MPP3 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: SQDDPTWWQAKRVGDTNLRAGLIPSKGFQERRLSYRRAAGTLPSPQSLRKPPYDQPCDKETCDCEGYLKGHYVAGL
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MPP3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
MPP3 Protein (NBP1-85014PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%), Rat (89%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for MPP3 Antibody

  • Discs large homolog 3
  • discs, large homolog 3
  • DLG3MAGUK p55 subfamily member 3
  • membrane protein palmitoylated 3
  • membrane protein, palmitoylated 3 (MAGUK p55 subfamily member 3)
  • Protein MPP3

Background

MPP3 product is a member of a family of membrane-associated proteins termed MAGUKs (membrane-associated guanylate kinase homologs). MAGUKs interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intracellular junctions. This protein contains a conserved sequence, called the SH3 (src homology 3) motif, found in several other proteins that associate with the cytoskeleton and are suspected to play important roles in signal transduction. Alternatively spliced transcript variants have been identified. One transcript variant is experimentally supported, but it doesn't encode a protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol MPP3