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Mouse VEGF Quantikine ELISA Kit, 2 Plate

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Increased production of proinflammatory cytokines and VEGF in the plasma and chondrocytes in high fat diet (HFD)-feeding mice.(a~d) levels of plasma IL-6 (a), TNF-alpha (b), IL-1 beta (c) and VEGF (d) in the C57BL/6 ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Conjugate
HRP

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
HRP
PMMV00
HRP
SMMV00
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Mouse VEGF Quantikine ELISA Kit, 2 Plate Summary

Background
The Quantikine Mouse VEGF Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse VEGF in cell culture supernates, tissue homogenates, mouse serum, and plasma. It contains Sf 21-expressed mouse VEGF and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse VEGF accurately. Results obtained using natural mo...use VEGF showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse VEGF.
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Specificity
This kit recognizes both the 164 and 120 amino acid residue forms of mouse VEGF
Source
N/A
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
Vegfa

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
Interference observed with 1 or more available related molecules.
Publications
Read Publications using
MMV00 in the following applications:

  • IHC
    1 publication

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse VEGF Quantikine ELISA Kit, 2 Plate

  • MVCD1
  • VAS
  • vascular endothelial growth factor A
  • Vascular permeability factor
  • Vasculotropin
  • VEGF
  • VEGFA
  • VEGF-A
  • VEGFMGC70609
  • VPF
  • VPFvascular endothelial growth factor

Background

Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and in adults (1-3). It is a member of the PDGF family that is characterized by the presence of eight conserved cysteine residues in a cystine knot structure and the formation of anti-parallel disulfide-linked dimers (4). Alternately spliced isoforms of 120, 164 and 188 amino acids (aa) have been found in mice, while 121, 145, 165, 183, 189, and 206 aa isoforms have been identified in humans (2, 4). In humans, VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189 (3, 4). The same pattern may exist in mice. Isoforms other than VEGF120 and VEGF121 contain basic heparin-binding regions and are not freely diffusible (4). Mouse VEGF164 shares 97% aa sequence identity with corresponding regions of rat VEGF. It also shares 89% aa sequence identity with human and porcine VEGF, 88% with bovine VEGF, and 90% with feline, equine, and canine VEGF. VEGF is expressed in multiple cells and tissues including skeletal and cardiac muscle (5, 6), hepatocytes (7), osteoblasts (8), neutrophils (9), macrophages (10), keratinocytes (11), brown adipose tissue (12), CD34+ stem cells (13), endothelial cells (14), fibroblasts, and vascular smooth muscle cells (15). VEGF expression is induced by hypoxia and cytokines such as IL-1, IL-6, IL-8, Oncostatin M, and TNF-alpha (3, 4, 9, 16). The isoforms are differentially expressed during development and in the adult (3). 
VEGF dimers bind to two related receptor tyrosine kinases, VEGF R1 (also called Flt-1) and VEGF R2 (Flk-1/KDR), and induce their homodimerization and autophosphorylation (3, 4, 7, 17, 18). These receptors have seven extracellular immunoglobulin-like domains and an intracellular split tyrosine kinase domain. They are expressed on vascular endothelial cells and a range of non-endothelial cells. Although VEGF affinity is highest for binding to VEGF R1, VEGF R2 appears to be the primary mediator of VEGF angiogenic activity (3, 4). VEGF165 also binds the semaphorin receptor, neuropilin-1, which promotes complex formation with VEGF R2 (19). 
VEGF is best known for its role in vasculogenesis. During embryogenesis, VEGF regulates the proliferation, migration, and survival of endothelial cells (3, 4), thus regulating blood vessel density and size but playing no role in determining vascular patterns. VEGF promotes bone formation through osteoblast and chondroblast recruitment and is also a monocyte chemoattractant (20-22). In postnatal life, VEGF maintains endothelial cell integrity and is a potent mitogen for micro- and macro-vascular endothelial cells. In adults, VEGF functions mainly in wound healing and the female reproductive cycle (3). In diseased tissues, VEGF promotes vascular permeability. It is thus thought to contribute to tumor metastasis by promoting both extravasation and tumor angiogenesis (23, 24). Various strategies have been employed therapeutically to antagonize VEGF-mediated tumor angiogenesis (25). Circulating VEGF levels correlate with disease activity in autoimmune diseases such as rheumatoid arthritis, multiple sclerosis, and systemic lupus erythematosus (26).

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for VEGF (MMV00)(228)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Transgenic Mouse, Xenograft.

We have publications tested in 1 application: IHC.


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IHC
(1)
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Human
(7)
Mouse
(216)
Rat
(6)
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(3)
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(1)
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Showing Publications 1 - 10 of 228. Show All 228 Publications.
Publications using MMV00 Applications Species
Shaldam, MA;Mousa, MHA;Tawfik, HO;El-Dessouki, AM;Sharaky, M;Saleh, MM;Alzahrani, AYA;Moussa, SB;Al-Karmalawy, AA; Muti-target rationale design of novel substituted N-phenyl-2-((6-phenylpyridazin-3-yl)thio)acetamide candidates as telomerase/JAK1/STAT3/TLR4 inhibitors: In vitro and in vivo investigations Bioorganic chemistry 2024-09-24 [PMID: 39332072] (Mouse) Mouse
Zaninelli, TH;Fattori, V;Heintz, OK;Wright, KR;Bennallack, PR;Sim, D;Bukhari, H;Terry, KL;Vitonis, AF;Missmer, SA;Andrello, AC;Anchan, RM;Godin, SK;Bree, D;Verri, WA;Rogers, MS; Targeting NGF but not VEGFR1 or BDNF signaling reduces endometriosis-associated pain in mice Journal of advanced research 2024-08-12 [PMID: 39142441] (Mouse, Transgenic Mouse) Mouse, Transgenic Mouse
Niimi, T;Tanaka, T;Aoyagi, C;Onda, Y;Nagamitsu, S;Kodama, S; Co-culture of vascular endothelial cells enhances corticosterone production in steroid hormone-producing cells generated from adipose-derived mesenchymal stromal cells Scientific reports 2024-08-13 [PMID: 39138321] (Mouse) Mouse
Mann, CG;MacArthur, MR;Zhang, J;Gong, S;AbuSalim, JE;Hunter, CJ;Lu, W;Agius, T;Longchamp, A;Allagnat, F;Rabinowitz, J;Mitchell, JR;De Bock, K;Mitchell, SJ; Sulfur Amino Acid Restriction Enhances Exercise Capacity in Mice by Boosting Fat Oxidation in Muscle bioRxiv : the preprint server for biology 2024-07-01 [PMID: 39005372] (Mouse, Transgenic Mouse) Mouse, Transgenic Mouse
Shimoji, K;Nakashima, T;Masuda, T;Namba, M;Sakamoto, S;Yamaguchi, K;Horimasu, Y;Mimae, T;Miyamoto, S;Iwamoto, H;Fujitaka, K;Hamada, H;Okada, M;Hattori, N; Hypoxia-inducible factor 1? modulates interstitial pneumonia-mediated lung cancer progression Journal of translational medicine 2023-11-27 [PMID: 38012636] (Mouse) Mouse
Chen, TQ;Wei, XJ;Liu, HY;Zhan, SH;Yang, XJ; Telocyte-Derived Exosomes Provide an Important Source of Wnts That Inhibits Fibrosis and Supports Regeneration and Repair of Endometrium Cell transplantation 2023-11-25 [PMID: 38006220] (Mouse) Mouse
Li, R;Chen, B;Kubota, A;Hanna, A;Humeres, C;Hernandez, SC;Liu, Y;Ma, R;Tuleta, I;Huang, S;Venugopal, H;Zhu, F;Su, K;Li, J;Zhang, J;Zheng, D;Frangogiannis, NG; Protective effects of macrophage-specific integrin ?5 in myocardial infarction are associated with accentuated angiogenesis Nature communications 2023-11-20 [PMID: 37985764] (Mouse) Mouse
Shimizu, M;Yoshimatsu, G;Morita, Y;Tanaka, T;Sakata, N;Tagashira, H;Wada, H;Kodama, S; Rescue of murine hind limb ischemia via angiogenesis and lymphangiogenesis promoted by cellular communication network factor 2 Scientific reports 2023-11-16 [PMID: 37973852] (Mouse) Mouse
Mandakhbayar, N;Ji, Y;El-Fiqi, A;Patel, KD;Yoon, DS;Dashnyam, K;Bayaraa, O;Jin, G;Tsogtbaatar, K;Kim, TH;Lee, JH;Kim, HW; Double hits with bioactive nanozyme based on cobalt-doped nanoglass for acute and diabetic wound therapies through anti-inflammatory and pro-angiogenic functions Bioactive materials 2024-01-01 [PMID: 37637079] (Human, Mouse) Human, Mouse
Huang, Y;Fan, Y;Zhao, Z;Zhang, X;Tucker, K;Staley, A;Suo, H;Sun, W;Shen, X;Deng, B;Pierce, SR;West, L;Yin, Y;Emanuele, MJ;Zhou, C;Bae-Jump, V; Inhibition of CDK1 by RO-3306 Exhibits Anti-Tumorigenic Effects in Ovarian Cancer Cells and a Transgenic Mouse Model of Ovarian Cancer International journal of molecular sciences 2023-08-03 [PMID: 37569750] (Mouse) Mouse
Show All 228 Publications.

Reviews for VEGF (MMV00) (0)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for VEGF (MMV00). (Showing 1 - 1 of 1 FAQs).

  1. Why is the molecular weight of VEGF different from the similar antibody, for some companies the the molecular weight is 40KD)?
    • I can't comment on another company's antibody because I don't have any information about their products. I can tell you that VEGF is expressed in a variety of isoforms and is subject to various post-translational modifications that influence its apparent molecular weight in an SDS-PAGE gel compared to the theoretical molecular weight.

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Understanding ‘Y’ in Breast Cancer: Crucial Role of DNA/RNA-binding Protein YB-1 in the Development, Pre-Invasive, and Metastatic Phases
Jamshed Arslan, Pharm D, PhD In the United States, 1 in 8 women will be diagnosed with breast cancer in her lifetime.1 Despite the prevalence, cancer genesis is a mystery. The heterogeneity of cancers makes it diff...  Read full blog post.
Detecting HIF alpha and beyond: Best controls for hypoxia Western blot analysis
By Rosa Moreno, PhD. Detecting HIF alpha and beyond: Best controls for hypoxia Western blot analysisPhysiological low levels of oxygen induce normal hypoxic events across biological systems. This hypoxic state activ...  Read full blog post.
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By Jamshed Arslan, Pharm. D., PhD. Stroke    is a leading cause of mortality and morbidity worldwide. Cellular players – neurons, astrocytes, endothelial and stromal cells – involved in post-stroke repair t...  Read full blog post.
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By Yoskaly Lazo-Fernandez, PhD The mammalian target of rapamycin (mTOR) is a conserved serine/threonine kinase that, as a member of two distinct intracellular protein complexes, mTORC1 and mTORC2, regulates protein ...  Read full blog post.
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Proliferating cell nuclear antigen (PCNA) plays a crucial role in nucleic acid metabolism as it pertains to DNA replication and repair.  Most noted for its activation of subunits of DNA polymerase, it has also been found to interact with cell-cycl...  Read full blog post.
Showing 1-10 of 23 blog posts - Show all blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

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Bioinformatics

Gene Symbol Vegfa