![Western Blot: Mouse Serum Albumin Antibody [NBP2-26510] - Western Blot of Mouse Serum Albumin antibody. Lane 1: molecular weight. Lane 2: mouse Mouse Serum Albumin. Load: 0.1 ug.Primary antibody: MSA antibody at 1:1000 in 1% BSA-TTBS for overnight at 4C.Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:20,000 for 45 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting 30 min at 4C.Predicted/Observed size: 69 kDa, 72 kDa for MSA. Other band(s): none.](http://images.novusbio.com/fullsize/Mouse-Serum-Albumin-Antibody-Western-Blot-NBP2-26510-img0010.jpg "Western Blot: Mouse Serum Albumin Antibody [NBP2-26510] - Western Blot of Mouse Serum Albumin antibody. Lane 1: molecular weight. Lane 2: mouse Mouse Serum Albumin. Load: 0.1 ug.Primary antibody: MSA antibody at 1:1000 in 1% BSA-TTBS for overnight at 4C.Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:20,000 for 45 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting 30 min at 4C.Predicted/Observed size: 69 kDa, 72 kDa for MSA. Other band(s): none.")
| Reactivity | MuSpecies Glossary |
| Applications | WB, DB, ELISA, IHC, PAGE |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | Mouse Serum Albumin antibody was prepared from monospecific antiserum by immunoaffinity chromatography using purified mouse serum albumin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. Analysis by SDS-PAGE was used to determine that this preparation is substantially free of aggregates and shows a banding pattern consistent with purified Rabbit IgG. Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. |
| Immunogen | Mouse Serum Albumin Antibody was produced by repeated immunizations with mouse serum albumin. (Uniprot: P07724) |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | Alb |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This product has been tested by ELISA and western blot and is assayed against 1.0 ug of Mouse Serum Albumin in a standard ELISA using Peroxidase conjugated Affinity Purified Goat anti-Rabbit IgG [H&L] MX and TMB as a substrate for 30 minutes at room temperature. |
| Storage | Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Preservative | 0.01% Sodium Azide |
| Purity | Immunogen affinity purified |
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Secondary Antibodies |
Isotype Controls |
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![Western Blot: Mouse Serum Albumin Antibody [NBP2-26510] - Western Blot: Albumin Antibody [Biotin] [NB600-929] - Lane 1: Human Serum Albumin. Load: 50 ng per lane. Primary antibody: Rabbit anti-Human Serum Albumin Antibody Biotin Conjugated at 1:1,000 overnight at 4C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 66 kDa, 60 kDa for Human Serum Albumin. Image using the Biotin form of this antibody.](http://images.novusbio.com/fullsize/Mouse-Serum-Albumin-Antibody-Western-Blot-NBP2-26510-img0005.jpg "Western Blot: Mouse Serum Albumin Antibody [NBP2-26510] - Western Blot: Albumin Antibody [Biotin] [NB600-929] - Lane 1: Human Serum Albumin. Load: 50 ng per lane. Primary antibody: Rabbit anti-Human Serum Albumin Antibody Biotin Conjugated at 1:1,000 overnight at 4C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 66 kDa, 60 kDa for Human Serum Albumin. Image using the Biotin form of this antibody.")
![SDS-Page: Mouse Serum Albumin Antibody [NBP2-26510]](http://images.novusbio.com/fullsize/Mouse-Serum-Albumin-Antibody-SDS-Page-NBP2-26510-img0007.jpg "SDS-Page: Mouse Serum Albumin Antibody [NBP2-26510]")
![SDS-Page: Mouse Serum Albumin Antibody [NBP2-26510] - SDS-PAGE of Mouse Mouse Serum Albumin antibody.Lane 1: mouse serum albumin reduced. Lane 2: molecular weight. Lane 3: mouse serum albumin non-reduced. Load: 1.0 ug.Predicted/Observed size: Analysis by SDS-PAGE shows a banding pattern consistent with purified Rabbit IgG.Other band(s): none.](http://images.novusbio.com/fullsize/Mouse-Serum-Albumin-Antibody-SDS-Page-NBP2-26510-img0011.jpg "SDS-Page: Mouse Serum Albumin Antibody [NBP2-26510] - SDS-PAGE of Mouse Mouse Serum Albumin antibody.Lane 1: mouse serum albumin reduced. Lane 2: molecular weight. Lane 3: mouse serum albumin non-reduced. Load: 1.0 ug.Predicted/Observed size: Analysis by SDS-PAGE shows a banding pattern consistent with purified Rabbit IgG.Other band(s): none.")
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