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Mouse MFG-E8 Quantikine ELISA Kit

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Summary
Reactivity MuSpecies Glossary
Applications ELISA
Conjugate
HRP

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Mouse MFG-E8 Quantikine ELISA Kit Summary

Background
The Quantikine Mouse MFG-E8 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse MFG-E8 in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant mouse MFG-E8 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural mouse MFG-E8 showed dose-...response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse MFG-E8.
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Specificity
Natural and recombinant mouse MFG-E8
Source
N/A
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
Mfge8

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using MFGE80.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse MFG-E8 Quantikine ELISA Kit

  • BA46
  • Breast epithelial antigen BA46
  • EDIL1
  • hP47
  • HsT19888
  • lactadherin
  • Lactahedrin
  • Medin
  • MFG1
  • MFGE8
  • MFG-E8
  • MFGM
  • milk fat globule-EGF factor 8 protein
  • Milk fat globule-EGF factor 8
  • O-acetyl disialoganglioside synthase
  • OAcGD3S
  • SED1
  • SPAG10
  • sperm associated antigen 10
  • sperm surface protein hP47

Background

MFG-E8, originally identified as a product of lactating mammary glands, is also secreted by activated macrophages and immature dendritic cells. MFG-E8 functions as an opsonin by binding phosphatidylserine on apoptotic cells and enhancing their phagocytosis.

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Mouse MFG-E8
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MFGE80
Mouse MFG-E8 Quantikine ELISA Kit
Species: Mu
Applications: ELISA
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for MFG-E8 (MFGE80)(9)

We have publications tested in 3 confirmed species: Mouse, Rat, Transgenic Mouse.


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Mouse
(7)
Rat
(1)
Transgenic Mouse
(2)
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Showing Publications 1 - 9 of 9.
Publications using MFGE80 Applications Species
Sokolova, D;Ghansah, SA;Puletti, F;Georgiades, T;De Schepper, S;Zheng, Y;Crowley, G;Wu, L;Rueda-Carrasco, J;Koutsiouroumpa, A;Muckett, P;Freeman, OJ;Khakh, BS;Hong, S; Astrocyte-derived MFG-E8 facilitates microglial synapse elimination in Alzheimer's disease mouse models bioRxiv : the preprint server for biology 2024-09-01 [PMID: 39257734] (Transgenic Mouse) Transgenic Mouse
Chaung, W;Zhang, F;Ma, G;Yen, H;Jacob, A;Brenner, M;Wang, P; Development of human cell-expressed tag-free rhMFG-E8 as a radiation mitigator and a therapeutic for acute kidney injury Research square 2023-05-15 [PMID: 37293000] (Rat) Rat
D Han, D Lu, S Huang, J Pang, Y Wu, J Hu, X Zhang, Y Pi, G Zhang, J Wang Small extracellular vesicles from Ptpn1-deficient macrophages alleviate intestinal inflammation by reprogramming macrophage polarization via lactadherin enrichment Redox Biology, 2022-11-28;58(0):102558. 2022-11-28 [PMID: 36462232] (Mouse, Transgenic Mouse) Mouse, Transgenic Mouse
M Ikemoto-Ue, H Zhou, T Kurosawa, Y Yoshimoto, M Toyoda, N Kanazawa, T Nakazawa, M Morita, K Tsuchida, A Uezumi Increased MFG-E8 at neuromuscular junctions is an exacerbating factor for sarcopenia-associated denervation Aging Cell, 2021-12-24;0(0):e13536. 2021-12-24 [PMID: 34953020] (Mouse) Mouse
Y Lu, L Liu, J Pan, B Luo, H Zeng, Y Shao, H Zhang, H Guan, D Guo, C Zeng, R Zhang, X Bai, H Zhang, D Cai MFG-E8 regulated by miR-99b-5p protects against osteoarthritis by targeting chondrocyte senescence and macrophage reprogramming via the NF-&kappaB pathway Cell Death & Disease, 2021-05-25;12(6):533. 2021-05-25 [PMID: 34031369] (Mouse) Mouse
HF Bu, S Subramania, H Geng, X Wang, F Liu, PM Chou, C Du, IG De Plaen, XD Tan MFG-E8 Plays an Important Role in Attenuating Cerulein-Induced Acute Pancreatitis in Mice Cells, 2021-03-25;10(4):. 2021-03-25 [PMID: 33806041] (Mouse) Mouse
J Brelstaff, AM Tolkovsky, B Ghetti, M Goedert, MG Spillantin Living Neurons with Tau Filaments Aberrantly Expose Phosphatidylserine and Are Phagocytosed by Microglia Cell Rep, 2018-08-21;24(8):1939-1948.e4. 2018-08-21 [PMID: 30134156] (Mouse) Mouse
Uchiyama A, Yamada K, Ogino S, Yokoyama Y, Takeuchi Y, Udey M, Ishikawa O, Motegi S MFG-E8 regulates angiogenesis in cutaneous wound healing. Am J Pathol, 2014-05-15;184(7):1981-90. 2014-05-15 [PMID: 24838098] (Mouse) Mouse
The neuroprotective effects of milk fat globule-EGF factor 8 against oligomeric amyloid beta toxicity. J Neuroinflammation, 2012-06-28;9(0):148. 2012-06-28 [PMID: 22742657] (Mouse) Mouse

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Bioinformatics

Gene Symbol Mfge8