Home » MMP-7 » MMP-7 Antibodies » MMP-7 Antibody [Unconjugated]

MMP-7 Antibody [Unconjugated]

 (11 Publications)
 See All Related
Submit a Review

Images

 
(
Enlarge)
MMP‑7 was detected in perfusion fixed frozen sections of mouse small intestine using Goat Anti-Mouse MMP‑7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2967) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of cells in intestinal glands. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
MMP‑7 was detected in perfusion fixed frozen sections of mouse small intestine using Goat Anti-Mouse MMP‑7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2967) at 15 µg/mL overnight at 4 ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF2967-SP
Novus Biologicals offers 100% Guarantee

MMP-7 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse MMP‑7
Leu18-Leu264
Accession # AAA99983
Specificity
Detects mouse MMP-7 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human MMP‑7 is observed, and less than 2% cross-reactivity with recombinant mouse (rm) MMP-2, rmMMP-3, rmMMP-8, and rmMMP-9 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MMP7
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
Publications
Read Publications using
AF2967 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MMP-7 Antibody [Unconjugated]

  • EC 3.4.24
  • EC 3.4.24.23
  • Matrilysin
  • matrin
  • matrix metallopeptidase 7 (matrilysin, uterine)
  • matrix metalloproteinase 7 (matrilysin, uterine)
  • Matrix metalloproteinase-7
  • MMP7
  • MMP-7
  • MPSL1
  • Pump-1 protease
  • PUMP1
  • PUMP-1
  • uterine matrilysin
  • Uterine metalloproteinase

Background

Matrix metalloproteinases (MMPs) are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-7 (matrilysin) is expressed in epithelial cells of normal and diseased tissues, and is capable of digesting a large series of proteins of the extracellular matrix including collagen IV and X, gelatin, casein, laminin, aggrecan, entactin, elastin and versican (1). MMP-7 is implicated in the activation of other proteinases such as plasminogen, MMP-1, MMP-2, and MMP-9. In addition to its roles in connective tissue remodeling and cancer, MMP-7 also regulates intestinal alpha ‑defensin activation in innate host defense, releases tumor necrosis factor-alpha in a model of herniated disc resorption, and cleaves FasL to generate a soluble form in a model of prostate involution. Structurally, MMP-7 is the smallest of the MMPs and consists of two domains: a pro-domain that is cleaved upon activation and a catalytic domain containing the zinc-binding site.

  1. Woessner, J.F. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al. eds. p. 532.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

MMP-2 [HRP]
MMP200
N/A MMP-2 [HRP]
N/A MMP-2 [HRP]
(102 Publications)
Species: Ca, Hu, Mu, Po, Rt
Applications: ELISA
MMP-9 [HRP]
DMP900
N/A MMP-9 [HRP]
N/A MMP-9 [HRP]
(220 Publications)
Species: Hu
Applications: ELISA
TIMP-1 [HRP]
DTM100
N/A TIMP-1 [HRP]
N/A TIMP-1 [HRP]
(97 Publications)
Species: Hu
Applications: ELISA
MMP-3 [HRP]
DMP300
N/A MMP-3 [HRP]
N/A MMP-3 [HRP]
(55 Publications)
Species: Hu
Applications: ELISA
MMP-1 Antibody
(36665) [Unconjugated
MAB901
Knockout Validated MMP-1 Antibody (36665) [Unconjugated]
Immunohistochemistry MMP-1 Antibody (36665) [Unconjugated]
Immunohistochemistry MMP-1 Antibody (36665) [Unconjugated]
(40 Publications)
Species: Hu
Applications: IHC, IP, KO, Neut, WB
TIMP-2 [HRP]
DTM200
N/A TIMP-2 [HRP]
N/A TIMP-2 [HRP]
N/A TIMP-2 [HRP]
(36 Publications)
Species: Hu
Applications: ELISA
MMP-14/MT1-MMP
Antibody (128527) [Un ...
MAB9181
Flow Cytometry MMP-14/MT1-MMP Antibody (128527) [Unconjugated]
Immunohistochemistry MMP-14/MT1-MMP Antibody (128527) [Unconjugated]
(11 Publications)
Species: Hu
Applications: CyTOF-ready, Flow, IHC, IP, WB
MMP-13 [HRP]
DM1300
N/A MMP-13 [HRP]
N/A MMP-13 [HRP]
(34 Publications)
Species: Hu
Applications: ELISA
MMP-12 Antibody
(SR03-23)
NBP2-67344
Flow Cytometry MMP-12 Antibody (SR03-23)
Immunohistochemistry-Paraffin MMP-12 Antibody (SR03-23)
Immunohistochemistry-Paraffin MMP-12 Antibody (SR03-23)
(9 Publications)
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
c-jun [p Ser63]
Antibody (1018Y) [Unc ...
MAB8930
Western Blot c-jun [p Ser63] Antibody (1018Y) [Unconjugated]
Immunocytochemistry c-jun [p Ser63] Antibody (1018Y) [Unconjugated]
Simple Western c-jun [p Ser63] Antibody (1018Y) [Unconjugated]
(1 Review)
(3 Publications)
Species: Hu
Applications: ICC, Simple Western, WB
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(821 Publications)
Species: Hu
Applications: BA
VEGF [HRP]
DVE00
N/A VEGF [HRP]
N/A VEGF [HRP]
N/A VEGF [HRP]
(685 Publications)
Species: Hu
Applications: ELISA
MMP-8
908-MP
MMP-8
(10 Publications)
Species: Hu
Applications: EnzAct
beta-Catenin
Antibody [Unconjugate
AF1329
Western Blot beta-Catenin Antibody [Unconjugated]
Chromatin Immunoprecipitation (ChIP) beta-Catenin Antibody [Unconjugated]
Simple Western beta-Catenin Antibody [Unconjugated]
(2 Reviews)
(33 Publications)
Species: Hu, Mu, Rt
Applications: ChIP, CyTOF-ready, ICC, IHC, ICFlow, Simple Western, WB
EGFR Antibody
[Unconjugated]
AF231
Immunohistochemistry EGFR Antibody [Unconjugated]
Immunocytochemistry EGFR Antibody [Unconjugated]
Western Blot EGFR Antibody [Unconjugated]
(30 Publications)
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, ICC, IHC, IP, Simple Western, WB
MMP-10 [HRP]
DM1000
N/A MMP-10 [HRP]
N/A MMP-10 [HRP]
(14 Publications)
Species: Hu
Applications: ELISA

Publications for MMP-7 Antibody (AF2967)(11)

We have publications tested in 2 confirmed species: Mouse, Transgenic Mouse.

We have publications tested in 4 applications: IHC, IHC-Fr, Neutralization, Western Blot.


Filter By Application
IHC
(4)
IHC-Fr
(2)
Neutralization
(1)
Western Blot
(1)
All Applications
Filter By Species
Mouse
(6)
Transgenic Mouse
(1)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using AF2967 Applications Species
E Murray, X Cheng, A Krishna, X Jin, TE Ohara, TS Stappenbec, R Bose HER2 and APC Mutations Promote Altered Crypt-Villus Morphology and Marked Hyperplasia in the Intestinal Epithelium Cellular and Molecular Gastroenterology and Hepatology, 2021-04-27;0(0):. 2021-04-27 [PMID: 33930605] (IHC, Transgenic Mouse) IHC Transgenic Mouse
Soledad A Camolotto, Shrivatsav Pattabiraman, Timothy L Mosbruger, Alex Jones, Veronika K Belova, Grace Orstad et al. FoxA1 and FoxA2 drive gastric differentiation and suppress squamous identity in NKX2-1-negative lung cancer eLife 2018-11-26 [PMID: 30475207]
YM Nusse, AK Savage, P Marangoni, AKM Rosendahl-, TA Landman, FJ de Sauvage, RM Locksley, OD Klein Parasitic helminths induce fetal-like reversion in the intestinal stem cell niche Nature, 2018-06-27;559(7712):109-113. 2018-06-27 [PMID: 29950724] (IHC, Mouse) IHC Mouse
K Nalaparedd, KJ Nattamai, RS Kumar, R Karns, KA Wikenheise, LL Sampson, MM Mahe, N Sundaram, MB Yacyshyn, B Yacyshyn, MA Helmrath, Y Zheng, H Geiger Canonical Wnt Signaling Ameliorates Aging of Intestinal Stem Cells Cell Rep, 2017-03-14;18(11):2608-2621. 2017-03-14 [PMID: 28297666] (IHC, Mouse) IHC Mouse
Haim Belinson, Adam K. Savage, Douglas Fadrosh, Yien-Ming Kuo, Din Lin, Ricardo Valladares et al. Dual epithelial and immune cell function of Dvl1 regulates gut microbiota composition and intestinal homeostasis JCI Insight 2016-07-07 [PMID: 27525310]
Interleukin-17 promotes prostate cancer via MMP7-induced epithelial-to-mesenchymal transition Oncogene, 2016-07-04;0(0):. 2016-07-04 [PMID: 27375020] (IHC-Fr, Mouse) IHC-Fr Mouse
Zhou Y, Rychahou P, Wang Q et al. TSC2/mTORC1 signaling controls Paneth and goblet cell differentiation in the intestinal epithelium Cell Death Dis. 2/6/2015 [PMID: 25654764]
Takwi Nkyimbeng, Clemens Ruppert, Takayuki Shiomi, Bhola Dahal, György Lang, Werner Seeger et al. Pivotal Role of Matrix Metalloproteinase 13 in Extracellular Matrix Turnover in Idiopathic Pulmonary Fibrosis PLoS ONE 2013-09-02 [PMID: 24023851]
Zhang Q, Liu S, Ge D, Zhang Q, Xue Y, Xiong Z, Abdel-Mageed A, Myers L, Hill S, Rowan B, Sartor O, Melamed J, Chen Z, You Z Interleukin-17 promotes formation and growth of prostate adenocarcinoma in mouse models. Cancer Res, 2012-03-28;72(10):2589-99. 2012-03-28 [PMID: 22461511] (IHC, Mouse) IHC Mouse
Littlepage LE, Sternlicht MD, Rougier N Matrix metalloproteinases contribute distinct roles in neuroendocrine prostate carcinogenesis, metastasis, and angiogenesis progression. Cancer Res., 2010-03-09;70(6):2224-34. 2010-03-09 [PMID: 20215503] (IHC-Fr, Western Blot, Mouse) IHC-Fr, Western Blot Mouse
Show All 11 Publications.

Reviews for MMP-7 Antibody (AF2967) (0)

There are no reviews for MMP-7 Antibody (AF2967). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for MMP-7 Antibody (AF2967) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional MMP-7 Products

Blogs on MMP-7.

Check out the latest blog posts on MMP-7.
Toll-like receptors in the intestinal epithelial cells
By Jamshed Arslan, Pharm. D., PhD. Toll-like receptors (TLRs) are microbe-sensing proteins that act as first responders to danger signals. TLRs help the intestinal epithelial cells (IECs) recognize commensal bacteria ...  Read full blog post.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

SH-SY5Y human neuroblastoma cell line was stained with Goat Anti-Human/Mouse/RatEphrin-B2 Antigen Affinity-purified Polyclonal Antibody (Catalog #AF496,filled histogram) or Normal Goat IgG Isotype Control Antibody(Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugatedAnti-Goat IgG Secondary Antibody (Catalog #F0107).
Goat IgG Isotype Control [Unconjugated]
AB-108-C
1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
210-TA-005

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our MMP-7 Antibody [Unconjugated] and receive a gift card or discount.


Bioinformatics

Gene Symbol MMP7
Uniprot