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MMP-13 Antibody [Unconjugated]

 (4 Publications)
 (1 Review)
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MMP-13 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using 15 µg/mL Goat Anti-Human MMP-13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF511) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
MMP-13 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using 15 µg/mL Goat Anti-Human MMP-13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF511) overnight at 4 °C. ...read more
MMP-13 was detected in immersion fixed paraffin-embedded sections of human breast array using Goat Anti-Human MMP-13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF511) at 15 µg/mL overnight at 4 °C. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF511
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF511-SP
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MMP-13 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human MMP-13
Leu20-Cys471
Accession # P45452
Specificity
Detects human MMP-13 in Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) MMP-3 and rhMMP-8 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MMP13
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.1 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AF511 in the following applications:
Publications
Read Publications using
AF511 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MMP-13 Antibody [Unconjugated]

  • CLG 3
  • CLG3EC 3.4.24
  • collagenase 3
  • EC 3.4.24.-
  • EC 3.4.24.22
  • EC 3.4.24.24
  • EC 3.4.24.35
  • EC 3.4.24.65
  • EC 3.4.24.7
  • MANDP1
  • matrix metallopeptidase 13 (collagenase 3)
  • matrix metalloproteinase 13 (collagenase 3)
  • Matrix metalloproteinase-13
  • MMP13
  • MMP-13

Background

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-13 (Collagenase-3) has been demonstrated to degrade a range of extracellular matrix proteins, including collagen types I, II, III, IV, IX, X and XIV, gelatin, aggrecan, perlecan and fibronectin. MMP-13 is distinguished from the other human collagenases by its effecient degradation of type II collagen. MMP-13 is expressed by fibroblasts, chrondrocytes and squamous epithelial cells. Structurally, MMP-13 may be divided into several distinct domains; a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a short hinge region and a carboxyl terminal (hemopexin-like) domain.

  1. Jeffery, J.J. (1998) in Collagenase 3. A.J. Barrett, et al. (eds): Handbook of Proteolytic Enzymes, San Diego: Academic Press, p. 1167.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MMP-13 Antibody (AF511)(4)

We have publications tested in 2 confirmed species: Human, Rabbit.

We have publications tested in 3 applications: ELISA Development, IHC, Western Blot.


Filter By Application
ELISA Development
(1)
IHC
(1)
Western Blot
(3)
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Human
(3)
Rabbit
(1)
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Showing Publications 1 - 4 of 4.
Publications using AF511 Applications Species
Yong HY, Kim IY, Kim JS, Moon A ErbB2-enhanced invasiveness of H-Ras MCF10A breast cells requires MMP-13 and uPA upregulation via p38 MAPK signaling. Int. J. Oncol., 2010-02-01;36(2):501-7. 2010-02-01 [PMID: 20043086] (Western Blot, Human) Western Blot Human
Nah SS, Choi IY, Yoo B, Kim YG, Moon HB, Lee CK Advanced glycation end products increases matrix metalloproteinase-1, -3, and -13, and TNF-alpha in human osteoarthritic chondrocytes. FEBS Lett., 2007-04-09;581(9):1928-32. 2007-04-09 [PMID: 17434489] (Western Blot, Human) Western Blot Human
Olivotto E, Vitellozzi R, Fernandez P, Falcieri E, Battistelli M, Burattini S, Flamigni F, Santi S, Borzi RM Chondrocyte hypertrophy and apoptosis induced by GROalpha require three-dimensional interaction with the extracellular matrix and a co-receptor role of chondroitin sulfate and are associated with the mitochondrial splicing variant of cathepsin B. J. Cell. Physiol., 2007-02-01;210(2):417-27. 2007-02-01 [PMID: 17096385] (ELISA Development, IHC, Human) ELISA Development, IHC Human
Pillinger MH, Rosenthal PB, Tolani SN, Apsel B, Dinsell V, Greenberg J, Chan ES, Gomez PF, Abramson SB Cyclooxygenase-2-derived E prostaglandins down-regulate matrix metalloproteinase-1 expression in fibroblast-like synoviocytes via inhibition of extracellular signal-regulated kinase activation. J. Immunol., 2003-12-01;171(11):6080-9. 2003-12-01 [PMID: 14634122] (Western Blot, Rabbit) Western Blot Rabbit

Review for MMP-13 Antibody (AF511) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF511:
Filter by Applications
WB
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
 WB Human 01/08/2015
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Summary

ApplicationWestern Blot
Sample TestedSee PMID 22521401
SpeciesHuman

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 Verified Customer
01/08/2015
Application: WB
Species: Human
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Bioinformatics

Gene Symbol MMP13
Uniprot