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MMP-10 Antibody [Unconjugated]

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MMP‑10 was detected in immersion fixed paraffin-embedded sections of human placenta using Human MMP‑10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF910) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
MMP‑10 was detected in immersion fixed paraffin-embedded sections of human placenta using Human MMP‑10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF910) at 5 µg/mL overnight at 4 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF910
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF910-SP
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MMP-10 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human MMP-10 (R&D Systems, Catalog # 910-MP)
Tyr18-Cys476
Accession # P09238
Specificity
Detects human MMP-10 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) MMP‑3 and rhMMP‑7 is observed under non-reducing conditions. Approximately 10% and 2% cross-reactivity with rhMMP-3 and rhMMP-2 is observed under reducing conditions, respectively. Additionally, less than 1% cross-reactivity with rhMMP-1, -8, -9, -12, -13, and recombinant mouse MMP-9 is observed under reducing and non-reducing conditions.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MMP10
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publication using AF910.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MMP-10 Antibody [Unconjugated]

  • EC 3.4.24
  • EC 3.4.24.22
  • matrix metallopeptidase 10 (stromelysin 2)
  • matrix metalloprotease 10
  • matrix metalloproteinase 10 (stromelysin 2)
  • Matrix metalloproteinase-10
  • MMP10
  • MMP-10
  • SL-2
  • STMY2stromelysin-2
  • Stromelysin 2
  • transin 2
  • transin-2

Background

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-10 (stromelysin 2) degrades a broad range of substrates including gelatin, collagen types III, IV and V, fibronectin, aggrecan, and pig cartilage proteoglycan. MMP-10 can activate other MMPs such as MMP-1 and MMP-8. MMP-10 is expressed in keratinocytes, T cells, menstrual endometrium, and a few tumor samples. Structurally, MMP-10 may be divided into four distinct domains: a pro-domain which is cleaved upon activation, a catalytic domain containing the zinc binding site, a short linker region, and a carboxyl terminal hemopexin-like domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MMP-10 Antibody (AF910)(1)


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Bioinformatics

Gene Symbol MMP10
Uniprot