MLKL Antibody (3B2)

Images

 
Immunocytochemistry/ Immunofluorescence: MLKL Antibody (3B2) [H00197259-M02] - Analysis of monoclonal antibody to MLKL on HeLa cell . Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: MLKL Antibody (3B2) [H00197259-M02] - Analysis of monoclonal antibody to MLKL on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.
ELISA: MLKL Antibody (3B2) [H00197259-M02] - Detection limit for recombinant GST tagged MLKL is 0.3 ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clone
3B2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

MLKL Antibody (3B2) Summary

Immunogen
MLKL (NP_689862, 371 a.a. ~ 471 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.VKSTAYLSPQELEDVFYQYDVKSEIYSFGIVLWEIATGDIPFQGCNSEKIRKLVAVKRQQEPLGEDCPSELREIIDECRAHDPSVRPSVDEILKKLSTFSK
Specificity
MLKL - mixed lineage kinase domain-like (3B2)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MLKL
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:500
Application Notes
Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.
Publications
Read Publications using
H00197259-M02 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MLKL Antibody (3B2)

  • FLJ34389
  • mixed lineage kinase domain-like protein
  • mixed lineage kinase domain-like
  • MLKL

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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H00197259-M02
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Applications: WB, ELISA, ICC/IF, IHC

Publications for MLKL Antibody (H00197259-M02)(4)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MLKL Antibody (H00197259-M02) (0)

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Secondary Antibodies

 

Isotype Controls

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Research Areas for MLKL Antibody (H00197259-M02)

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Blogs on MLKL.

Rest in Peace: Is the Receptor Interacting Protein (RIP) Kinase-3 (RIPK3) a Protector or Offender?
By Jamshed Arslan, Pharm. D., PhD. Receptor interacting protein kinases (RIPKs) in necroptosisDeath is perhaps inevitable. Cell death can be programed and immunologically silent (apoptosis), unprogrammed and inflamm...  Read full blog post.

Apoptosis and Necroptosis Part II: Inhibitors of apoptosis proteins (IAPs); Key regulators of the balance between necroptosis, apoptosis and survival
In the first installment of this two-part blog post titled "Apoptosis and Necroptosis: Important factors to identify both types of programmed cell death", the mechanisms by which cell death occurs and ways to identify these pathways were ...  Read full blog post.

Apoptosis and Necroptosis Part I: Important factors to identify both types of programmed cell death
Different types of cell death have classically been identified by discrete morphological changes. The hallmarks of apoptosis include cell shrinkage, nuclear fragmentation and membrane blebbing whereas necroptosis is characterized by cell swelling ...  Read full blog post.

Read our latest blog and use the new citation tool on bio-techne.com

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Bioinformatics

Gene Symbol MLKL
Entrez
Uniprot