MLH1 Antibody (S07-5D3)

Images

 
Western Blot: MLH1 Antibody (S07-5D3) [NBP3-14898] - Western blot detection of MLH1 in Hela, A549, HL-60, U2OS cell lysates using MLH1 Rabbit mAb (1:1000 diluted). Predicted band size: 85kDa. Observed band size: 85kDa.
Immunocytochemistry/ Immunofluorescence: MLH1 Antibody (S07-5D3) [NBP3-14898] - Immunocytochemistry of MLH1 (green) in hela using MLH1 Rabbit mAb at dilution 1/50, and DAPI (blue)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clone
S07-5D3
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

MLH1 Antibody (S07-5D3) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
Recombinant protein of human MLH1 (Uniprot#: P40692)
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
MLH1
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1/1000
Theoretical MW
85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
Preservative
0.01% Sodium Azide
Purity
Affinity purified

Alternate Names for MLH1 Antibody (S07-5D3)

  • COCA2FCC2
  • DNA mismatch repair protein Mlh1
  • hMLH1
  • HNPCC
  • HNPCC2MGC5172
  • mutL (E. coli) homolog 1 (colon cancer, nonpolyposis type 2)
  • mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli)
  • MutL protein homolog 1

Background

MLH1 is a DNA mismatch repair protein. The repair of mismatch DNA is essential to maintaining the integrity of genetic information over time. An alteration of microsatellite repeats is the result of slippage owing to strand misalignment during DNA replication and is referred to as microsatellite instability (MSI). These defects in DNA repair pathways have been related to human carcinogenesis. The importance of mismatch repair genes became apparent with the identification of the genetic basis for hereditary nonpolyposis colon cancer (HNPCC). MSHS2 is involved in the initial cognition of mismatch nucleotides during the replication mismatch repair process. It is thought that after MSH2 binds to a mismatched DNA duplex it is joined by a heterodimer of MLH1 and PMSH, which together help facilitate the later steps in mismatch repair.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MLH1 Antibody (NBP3-14898) (0)

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Secondary Antibodies

 

Isotype Controls

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Research Areas for MLH1 Antibody (NBP3-14898)

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Blogs on MLH1.

NER, BER, HR and Other DNA Repair Pathways
A large number of antibody assays are devoted to the study of nuclear excision repair (NER) proteins. However, there are a number of other DNA repair pathways, many of which are instigated by NER and share the same proteins. DNA repair antibodies are ...  Read full blog post.

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Bioinformatics

Gene Symbol MLH1