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Microsomal Glutathione S-transferase 1 Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: Microsomal Glutathione S-transferase 1 Antibody [NBP1-92116] - Staining in human liver and skeletal muscle tissues using anti-MGST1 antibody. Corresponding ...read more
Immunocytochemistry/ Immunofluorescence: Microsomal Glutathione S-transferase 1 Antibody [NBP1-92116] - Staining of human cell line A-431 shows localization to mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Microsomal Glutathione S-transferase 1 Antibody [NBP1-92116] - Staining of human liver shows high expression.
Immunohistochemistry-Paraffin: Microsomal Glutathione S-transferase 1 Antibody [NBP1-92116] - Staining of human skeletal muscle shows low expression as expected.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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Microsomal Glutathione S-transferase 1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: FYRLTRKVFANPEDCVAFGKGENAKKYLRTDDR
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MGST1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Microsomal Glutathione S-transferase 1 Protein (NBP1-92116PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Microsomal Glutathione S-transferase 1 Antibody

  • glutathione S-transferase 12
  • GST12EC 2.5.1.18
  • MGC14525
  • MGST
  • MGST-I
  • microsomal glutathione S-transferase 1
  • Microsomal GST-1
  • Microsomal GST-I

Background

The MAPEG (Membrane Associated Proteins in Eicosanoid and Glutathione metabolism) family consists of six human proteins, two of which are involved in the production of leukotrienes and prostaglandin E, important mediators of inflammation. Other family members, demonstrating glutathione S-transferase and peroxidase activities, are involved in cellular defense against toxic, carcinogenic, and pharmacologically active electrophilic compounds. This gene encodes a protein that catalyzes the conjugation of glutathione to electrophiles and the reduction of lipid hydroperoxides. This protein is localized to the endoplasmic reticulum and outer mitochondrial membrane where it is thought to protect these membranes from oxidative stress. Four transcript variants of this gene encode one protein isoform. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MGST1