Human Metallothionein-1A ELISA Kit (Colorimetric) Summary
Specificity |
This assay has high sensitivity and excellent specificity for detection of human MT. No significant cross-reactivity or interference between human MT and analogues was observed. |
Standard Curve Range |
6.25 pg/mL - 400 pg/mL |
Sensitivity |
0.813 pg/mL |
Assay Type |
Sandwich ELISA |
Inter-Assay |
%CV < 10 |
Intra-Assay |
%CV < 8 |
Sample Volume |
50 - 100 uL |
Kit Type |
ELISA Kit (Colorimetric) |
Gene |
MT1A |
Applications/Dilutions
Packaging, Storage & Formulations
Kit Components
Components
|
- Adhesive Strip (For 96 wells)
- Assay plate (12 x 8 coated Microwells)
- Biotin-antibody (100 x concentrate)
- Biotin-antibody Diluent
- HRP-avidin (100 x concentrate)
- HRP-avidin Diluent
- Instruction manual
- Sample Diluent
- Standard (Freeze dried)
- Stop Solution
- TMB Substrate
- Wash Buffer (25 x concentrate)
|
Alternate Names for Human Metallothionein-1A ELISA Kit (Colorimetric)
Background
This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MT is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MT bound in the initial step. The color development is stopped and the intensity of the color is measured.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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