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Human Metallothionein-1A ELISA Kit (Colorimetric)

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ELISA: Human metallothionein ELISA Kit (Colorimetric) [NBP2-60092]
Human Metallothionein-1A ELISA Kit (Colorimetric) [NBP2-60092] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, cell culture supernates, tissue homogenates, urine and saliva
Standard Curve Range
6.25 pg/mL - 400 pg/mL
Sensitivity
0.813 pg/mL

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Human Metallothionein-1A ELISA Kit (Colorimetric) Summary

Specificity
This assay has high sensitivity and excellent specificity for detection of human MT. No significant cross-reactivity or interference between human MT and analogues was observed.
Standard Curve Range
6.25 pg/mL - 400 pg/mL
Sensitivity
0.813 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 10
Intra-Assay
%CV < 8
Sample Volume
50 - 100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
MT1A

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Store at 4°C.

Kit Components

Components
  1. Adhesive Strip (For 96 wells)
  2. Assay plate (12 x 8 coated Microwells)
  3. Biotin-antibody (100 x concentrate)
  4. Biotin-antibody Diluent
  5. HRP-avidin (100 x concentrate)
  6. HRP-avidin Diluent
  7. Instruction manual
  8. Sample Diluent
  9. Standard (Freeze dried)
  10. Stop Solution
  11. TMB Substrate
  12. Wash Buffer (25 x concentrate)

Alternate Names for Human Metallothionein-1A ELISA Kit (Colorimetric)

  • metallothionein 1A (functional)
  • metallothionein 1A
  • metallothionein 1S
  • metallothionein-1A
  • metallothionein-IA
  • MGC32848
  • MT1
  • MT-1A
  • MT1S
  • MTC
  • MT-IA

Background

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MT is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MT bound in the initial step. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol MT1A