![Western Blot: Melanophilin Antibody [NB300-943] - Staining (0.5 ug/mL) of total melanocyte PNS (200 ug per lane) of wild-type and leaden (Mlph null) mice. The upper band may be the stacking-separating gel interface. Data kindly provided by A N Hume, Imperial College](http://images.novusbio.com/fullsize/Melanophilin-Antibody-Western-Blot-NB300-943-img0003.jpg "Western Blot: Melanophilin Antibody [NB300-943] - Staining (0.5 ug/mL) of total melanocyte PNS (200 ug per lane) of wild-type and leaden (Mlph null) mice. The upper band may be the stacking-separating gel interface. Data kindly provided by A N Hume, Imperial College")
| Reactivity | Mu, RtSpecies Glossary |
| Applications | WB, ELISA, ICC/IF |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence C-ARHIFAKPVMAQQP corresponding to C-Terminus according to Uniprot Mouse NP_443748.2. |
| Predicted Species | Rat (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | MLPH |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | IF successful usage for stained mouse melanocytes is reported in scientific literature (PMID: 18559336). GST-pull down assay usage is reported in scientific literature(PMID: 18559336). WB: Approx. 80 kDa band observed in mouse Melanocyte extracts. Please note that this product may require overnight incubation of primary and high protein loads. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | MLPH |
| Entrez | |
| Uniprot |
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![Immunocytochemistry/Immunofluorescence: Melanophilin Antibody [NB300-943] - Wild-type C57bl6 derived melanocytes were stained with (0.5 ug/mL) and detected with Alexa568 conjugated donkey anti-sheep second antibodies. Brightfield image shows melanosome distribution. Data kindly provided by A N Hume, Imperial College.](http://images.novusbio.com/fullsize/Melanophilin-Antibody-Immunohistochemistry-Paraffin-NB300-943-img0002.jpg "Immunocytochemistry/Immunofluorescence: Melanophilin Antibody [NB300-943] - Wild-type C57bl6 derived melanocytes were stained with (0.5 ug/mL) and detected with Alexa568 conjugated donkey anti-sheep second antibodies. Brightfield image shows melanosome distribution. Data kindly provided by A N Hume, Imperial College.")
