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MEKK3 Antibody (576240) [Unconjugated]

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Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Daudi human Burkitt's lymphoma cell line, and A431 human epithelial carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human MEKK3 Monoclonal Antibody (Catalog # MAB6095) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for MEKK3 at approximately 71 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Daudi human Burkitt's lymphoma cell line, and A431 human epithelial carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Mouse ...read more
Genetic Strategies: Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and MEKK3 knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, KO
Clone
576240
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

    

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB6095-SP
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MEKK3 Antibody (576240) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human MEKK3
Ser43-Tyr275
Accession # Q99759
Specificity
Detects human MEKK3 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MAP3K3
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Knockout Validated
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MEKK3 Antibody (576240) [Unconjugated]

  • EC 2.7.11.25
  • MAP/ERK kinase kinase 3
  • MAP3K3
  • MAPK/ERK kinase kinase 3
  • MAPKKK3
  • MAPKKK3MEKK 3
  • MEK kinase 3
  • MEKK3
  • mitogen-activated protein kinase kinase kinase 3

Background

MAPK/ERK kinase kinase 3 (MEKK3), also known as MEK kinase 3 and MAP3K3, is a widely expressed 71 kDa Ser/Thr kinase in the SEK, MEK, and MyD88‑IRAK‑TRAF6 signal transduction pathways. It contains a PB1 domain (aa 45‑123), a region with several phosphorylation sites, and a protein kinase domain (aa 361‑621). MEKK3 is involved in tissue morphogenesis, inflammation, and the response to cellular stress. Its activation leads to the activation of NF kappa B following stimulation of IL-1 R, TLR4, TLR8, TNF RI, and the AT1 angiotensin receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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MEKK3 Antibody
(576240) [Unconjugate
MAB6095
MEKK3 Antibody (576240) [Unconjugated]
Species: Hu
Applications: WB, KO

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Bioinformatics

Gene Symbol MAP3K3
Uniprot