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MEK1 Antibody (239421)

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Western blot shows lysates of U937 human histiocytic lymphoma cell line, HepG2 human hepatocellular carcinoma cell line, DU145 human prostate carcinoma cell line, and TS1 mouse helper T cell line. PVDF membrane was probed with 0.5 µg/mL Mouse Anti-Human/ Mouse/Rat MEK1 Monoclonal Antibody (Catalog # MAB2079) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human MEK1 and Recombinant Human Active MEK2 (Catalog # 2855-KS) (5 ng/lane) were included. A specific band for MEK1 was detected at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Western blot shows lysates of U937 human histiocytic lymphoma cell line, HepG2 human hepatocellular carcinoma cell line, DU145 human prostate carcinoma cell line, and TS1 mouse helper T cell line. PVDF membrane was ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clone
239421
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB2079-SP
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MEK1 Antibody (239421) Summary

Immunogen
E. coli-derived recombinant human MEK1
Lys2-Val393 (Ser218Asp, Ser222Asp)
Accession # Q02750
Specificity
Detects human, mouse, and rat MEK1 in Western blots. In Western blots, no crossreactivity with recombinant human MEK2 is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
MAP2K1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MEK1 Antibody (239421)

  • dual specificity mitogen-activated protein kinase kinase 1
  • ERK activator kinase 1
  • MAP kinase kinase 1
  • MAP2K1
  • MAPK/ERK kinase 1
  • MAPKK1
  • MEK1
  • MEK1MAPKK 1
  • mitogen-activated protein kinase kinase 1
  • MKK1
  • MKK1MEK 1
  • PRKMK1EC 2.7.12.2
  • protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)

Background

Mitogen-Activated Protein Kinase Kinase 1 (MEK1 or MAP2K1), also known as MKK1, belongs to the family of STE kinases. Both MEK1 and the related MEK2 aredual‑specificity kinases, phosphorylating and activating the mitogen-activated protein kinases ERK1 and ERK2 at T and Y positions within the phosphoacceptor sequence T-E-Y. Activation of MEK1 by Raf occurs through phosphorylation at S218 and S222.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol MAP2K1
Uniprot