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Mannose Phosphate Isomerase Antibody

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Genetic Strategies: Knockout Validated: Mannose Phosphate Isomerase Antibody [NBP2-17215] - Wild-type (WT) and MPI knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was ...read more
Western Blot: Mannose Phosphate Isomerase Antibody [NBP2-17215] - Sample (30 ug of whole cell lysate) A: JurKat B: Hela 10% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: Mannose Phosphate Isomerase Antibody [NBP2-17215] - Immunohistochemical analysis of paraffin-embedded TOV21G xenograft, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications WB, IHC, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

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Mannose Phosphate Isomerase Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Mannose Phosphate Isomerase. The exact sequence is proprietary.
Localization
Cytoplasm
Predicted Species
Primate (91%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MPI
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Knockout Validated
  • Western Blot 1:500-1:3000
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chimpanzee (91%), Bovine (87%), Mouse (80%), Rat (82%), Pig (86%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for Mannose Phosphate Isomerase Antibody

  • CDG1B
  • EC 5.3.1
  • EC 5.3.1.8
  • FLJ39201
  • mannose phosphate isomerase
  • mannose-6- phosphate isomerase
  • mannose-6-phosphate isomerase
  • Phosphohexomutase
  • phosphomannose isomerase 1
  • Phosphomannose isomerase
  • PMI
  • PMI1phosphohexomutase

Background

Phosphomannose isomerase catalyzes the interconversion of fructose-6-phosphate and mannose-6-phosphate and plays a critical role in maintaining the supply of D-mannose derivatives, which are required for most glycosylation reactions. Mutations in the MPI gene were found in patients with carbohydrate-deficient glycoprotein syndrome, type Ib. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MPI
Uniprot