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MAdCAM-1 Antibody (OTI1A5) [Janelia Fluor® 669]

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Product Details

Summary
Reactivity Hu, PoSpecies Glossary
Applications WB, ICC/IF
Clone
OTI1A5
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 669

Order Details

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MAdCAM-1 Antibody (OTI1A5) [Janelia Fluor® 669] Summary

Immunogen
Full length human recombinant protein of human MADCAM1(NP_570116) produced in HEK293T cell.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
MADCAM1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for MAdCAM-1 Antibody (OTI1A5) [Janelia Fluor® 669]

  • hMAdCAM-1
  • MACAM1
  • MAdCAM1
  • MAdCAM-1
  • mucosal addressin cell adhesion molecule 1
  • mucosal addressin cell adhesion molecule-1
  • mucosal vascular addressin cell adhesion molecule 1

Background

Mucosal vascular addressin-1 (MAdCAM-1) is a type I transmembrane glycoprotein expressed at high levels on high endothelial venules (HEV) of Peyer's patches and mesenteric lymph nodes, and on flat walled venules within the gut lamina propria. It is also expressed on sinus-lining cells in the spleen. (1-3) The countereceptor or "homing receptor" for MAdCAM-1 is alpha4beta7 integrin (also known as LPAM-1). MAdCAM-1 is also a facultative ligand for CD62L/L-selectin. (4, 5) The monoclonal antibody MECA-367 binds to the first domain of MAdCAM-1 and blocks MAdCAM-1-dependent binding in vitro and lymphocyte homing to Peyer's patch HEV in vivo. (1, 2, 4)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol MADCAM1