Home » MAD4 » MAD4 Antibodies » MAD4 Antibody (OTI7F7) [DyLight 488]
Novus Biologicals products are now on bio-techne.com

MAD4 Antibody (OTI7F7) [DyLight 488]

 Submit a Review

Images

 
There are currently no images for MAD4 Antibody (NBP2-72567G).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
OTI7F7
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 488

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-72567AF350
Alexa Fluor 405
NBP2-72567AF405
Alexa Fluor 488
NBP2-72567AF488
Alexa Fluor 532
NBP2-72567AF532
Alexa Fluor 594
NBP2-72567AF594
Alexa Fluor 647
NBP2-72567AF647
Alexa Fluor 700
NBP2-72567AF700
Alexa Fluor 750
NBP2-72567AF750
Allophycocyanin
NBP2-72567APC
Biotin
NBP2-72567B
CoraFluor 1
NBP2-72567CL1
DyLight 350
NBP2-72567UV
DyLight 405
NBP2-72567V
DyLight 550
NBP2-72567R
DyLight 594
NBP2-72567DL594
DyLight 650
NBP2-72567C
DyLight 680
NBP2-72567FR
DyLight 755
NBP2-72567IR
FITC
NBP2-72567F
HRP
NBP2-72567H
Janelia Fluor 525
NBP2-72567JF525
Janelia Fluor 549
NBP2-72567JF549
Janelia Fluor 585
NBP2-72567JF585
Janelia Fluor 635
NBP2-72567JF635
Janelia Fluor 646
NBP2-72567JF646
Janelia Fluor 669
NBP2-72567JF669
PE
NBP2-72567PE
PerCP
NBP2-72567PCP
Unconjugated
NBP2-46060
mFluor Violet 450 SE
NBP2-72567MFV450
mFluor Violet 500 SE
NBP2-72567MFV500
mFluor Violet 610 SE
NBP2-72567MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-46060
Azide and BSA Free
NBP2-72567
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

MAD4 Antibody (OTI7F7) [DyLight 488] Summary

Immunogen
Full length recombinant protein of human MAD4 (NP_006445) produced in E.coli.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
MXD4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for MAD4 Antibody (OTI7F7) [DyLight 488]

  • BHLHC12
  • bHLHc12Max-interacting transcriptional repressor MAD4
  • Class C basic helix-loop-helix protein 12
  • Mad4 homolog
  • MAD4Max-associated protein 4
  • MAX dimerization protein 4
  • Max dimerizer 4
  • MST149
  • MSTP149
  • MXD4 MAX dimerization protein 4

Background

MAD4 is a member of the MAD gene family . The MAD genes encode basic helix loop helix leucine zipper proteins that heterodimerize with MAX protein, forming a transcriptional repression complex. The MAD proteins compete for MAX binding with MYC, which heterodimerizes with MAX forming a transcriptional activation complex. Studies in rodents suggest that the MAD genes are tumor suppressors and contribute to the regulation of cell growth in differentiating tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

c-Myc Antibody
(9E10) - BSA Free
NB600-302
Flow Cytometry c-Myc Antibody (9E10) - BSA Free
Flow Cytometry c-Myc Antibody (9E10) - BSA Free
Western Blot c-Myc Antibody (9E10) - BSA Free
(4 Reviews)
(46 Publications)
Species: Bv, Dr, Hu, Mu
Applications: ChIP, ELISA, Flow-IC, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, S-ELISA, Simple Western, WB
Mad Antibody
NBP2-85249
Western Blot Mad Antibody
Western Blot Mad Antibody
Western Blot Mad Antibody
Species: Hu, Mu
Applications: WB
Max Antibody
NB100-793
Immunohistochemistry-Paraffin Max Antibody
Immunofluorescence Max Antibody
Immunofluorescence Max Antibody
(3 Publications)
Species: Hu, Rt
Applications: ICC/IF, IHC, IHC-P, PEP-ELISA, WB
MAD1L1/MAD1
Antibody
NBP1-32775
Immunohistochemistry-Paraffin MAD1L1/MAD1 Antibody
Western Blot MAD1L1/MAD1 Antibody
Western Blot MAD1L1/MAD1 Antibody
(2 Publications)
Species: Hu, Mar, Mu, Rt
Applications: ChIP, ICC/IF, IHC, IHC-P, IP, WB
AMPD1 Antibody
NBP2-24509
Western Blot AMPD1 Antibody
Immunohistochemistry-Paraffin AMPD1 Antibody
(3 Publications)
Species: Bv, Ca, Hu, Mu, Ma-Op, Pm, Rt
Applications: IHC, IHC-P, WB
Mxi1 Antibody
[Unconjugated]
AF4185
Immunocytochemistry Mxi1 Antibody [Unconjugated]
(2 Publications)
Species: Hu
Applications: ICC
Smad3 Antibody
NB100-56479
Immunocytochemistry/ Immunofluorescence Smad3 Antibody
Western Blot Smad3 Antibody
Immunohistochemistry-Paraffin Smad3 Antibody
(5 Reviews)
(8 Publications)
Species: Bv, Ca, Hu, Mu, Po, Pm, Rt
Applications: ICC/IF, IHC, IHC-P, WB
IkB-alpha Antibody
(6A920) - BSA Free
NB100-56507
Knockout Validated IkB-alpha Antibody (6A920) - BSA Free
Flow Cytometry IkB-alpha Antibody (6A920) - BSA Free
Flow Cytometry IkB-alpha Antibody (6A920) - BSA Free
(42 Publications)
Species: Hu, Mu, Rt
Applications: CyTOF-ready, Flow, ICC/IF, IHC, IHC-P, IP, KO, Simple Western, WB
MAD3 Antibody
(S07-6H7)
NBP3-19989
Western Blot MAD3 Antibody (S07-6H7)
Species: Hu, Mu, Rt
Applications: WB
MAX binding
protein Antibody (HL2 ...
NBP3-25567
Western Blot MAX binding protein Antibody (HL2309) - Azide and BSA Free
Western Blot MAX binding protein Antibody (HL2309) - Azide and BSA Free
Immunohistochemistry-Paraffin MAX binding protein Antibody (HL2309) - Azide and BSA Free
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
MLX Antibody
[Unconjugated]
AF4186
Western Blot MLX Antibody [Unconjugated]
Immunocytochemistry MLX Antibody [Unconjugated]
(1 Publication)
Species: Hu
Applications: ICC, WB
Miz-1/ZBTB17
Antibody
AF3760
Western Blot Miz-1/ZBTB17 Antibody
(2 Publications)
Species: Hu
Applications: WB
Smad4 Antibody
[Unconjugated]
AF2097
Chromatin Immunoprecipitation (ChIP) Smad4 Antibody [Unconjugated]
Western Blot Smad4 Antibody [Unconjugated]
Immunocytochemistry Smad4 Antibody [Unconjugated]
(9 Publications)
Species: Hu
Applications: ChIP, ICC, WB
n-Myc Antibody
(NMYC-1) - BSA Free
NB200-109
Western Blot n-Myc Antibody (NMYC-1) - BSA Free
Western Blot n-Myc Antibody (NMYC-1) - BSA Free
Immunocytochemistry/ Immunofluorescence n-Myc Antibody (NMYC-1) - BSA Free
(6 Publications)
Species: Hu, Mu
Applications: ChIP, ChIP, CyTOF-ready, Flow, GS, ICC/IF, IP, WB
OX40/TNFRSF4
Antibody [Unconjugate
AF3388
Immunohistochemistry OX40/TNFRSF4 Antibody [Unconjugated]
Neutralization OX40/TNFRSF4 Antibody [Unconjugated]
Flow Cytometry OX40/TNFRSF4 Antibody [Unconjugated]
(1 Review)
(2 Publications)
Species: Hu
Applications: CyTOF-ready, Flow, IHC, Neut, WB
Ornithine
Decarboxylase Antibod ...
NBP2-32887
Immunohistochemistry-Paraffin Ornithine Decarboxylase Antibody (ODC1/485)
Immunocytochemistry/ Immunofluorescence Ornithine Decarboxylase Antibody (ODC1/485)
Western Blot Ornithine Decarboxylase Antibody (ODC1/485)
(1 Review)
(2 Publications)
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
BLIMP1/PRDM1
Antibody (3H2-E8) - B ...
NB600-235
Immunocytochemistry/ Immunofluorescence BLIMP1/PRDM1 Antibody (3H2-E8) - BSA Free
Flow Cytometry BLIMP1/PRDM1 Antibody (3H2-E8) - BSA Free
Immunocytochemistry/ Immunofluorescence BLIMP1/PRDM1 Antibody (3H2-E8) - BSA Free
(2 Reviews)
(52 Publications)
Species: Hu, Mu, Po
Applications: ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
CHREBP Antibody -
BSA Free
NB400-135
Knockout Validated CHREBP Antibody - BSA Free
Immunohistochemistry CHREBP Antibody - BSA Free
Western Blot CHREBP Antibody - BSA Free
(7 Reviews)
(151 Publications)
Species: Hu, Mu, Rt
Applications: ChIP, ChIP, GS, IB, ICC/IF, IHC, IHC-P, IP, KD, KO, PAGE, WB
p53 Antibody (PAb
240) - BSA Free
NB200-103
Flow Cytometry p53 Antibody (PAb 240) - BSA Free
Flow Cytometry p53 Antibody (PAb 240) - BSA Free
Flow (Intracellular) p53 Antibody (PAb 240) - BSA Free
(3 Reviews)
(42 Publications)
Species: Hu, Mu, Rt, Xp(-), Ye
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
MAD4 Antibody
(OTI7F7) [DyLight 488
NBP2-72567G
MAD4 Antibody (OTI7F7) [DyLight 488]
Species: Hu, Mu
Applications: WB, ICC/IF, IHC

Publications for MAD4 Antibody (NBP2-72567G) (0)

There are no publications for MAD4 Antibody (NBP2-72567G).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for MAD4 Antibody (NBP2-72567G) (0)

There are no reviews for MAD4 Antibody (NBP2-72567G). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MAD4 Antibody (NBP2-72567G) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional MAD4 Products

Array NBP2-72567G

Research Areas for MAD4 Antibody (NBP2-72567G)

Find related products by research area.

Blogs on MAD4

There are no specific blogs for MAD4, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Knockout Validated: CHREBP Antibody - BSA Free [NB400-135] - Immunoblot analysis of ChREBP in liver lysates of control and L-Chrebp -/- mice. Aliquots (60 ug of protein) of liver whole-cell lysates were subjected to SDS-PAGE and immunoblot analysis with anti-ChREBP and anti-calnexin antibodies. ChREBP delta denotes a truncated aberrant ChREBP protein present only in lysates prepared from L-Chrebp -/- livers. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29335275/) licensed under a CC-BY license.
CHREBP Antibody - BSA Free
NB400-135
Immunocytochemistry/Immunofluorescence: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-BLIMP1/PRDM1 (3H2-E8) conjugated to DyLight 550 [NB600-235R] at 20ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
BLIMP1/PRDM1 Antibody (3H2-E8) - BSA Free
NB600-235

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our MAD4 Antibody (OTI7F7) [DyLight 488] and receive a gift card or discount.


Bioinformatics

Gene Symbol MXD4