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MAD4 Antibody (OTI7F7)

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Western Blot: MAD4 Antibody (OTI7F7) [NBP2-46060] - HEK293T cells were transfected with the pCMV6- ENTRY control (Left lane) or pCMV6-ENTRY MXD4 cDNA for 48 hrs and lysed.Equivalent amounts of cell lysates (5 ug per ...read more
Immunocytochemistry/ Immunofluorescence: MAD4 Antibody (OTI7F7) [NBP2-46060] - Human keratinocytes, PFA-fixed, permabilisation: PBS-Triton 0.1% 20 minutes at RT, primary antibody at 1:250 in PBS-2% BSA, secondary ...read more
Immunohistochemistry-Paraffin: MAD4 Antibody (OTI7F7) [NBP2-46060] - Human tonsil within the normal limits using anti-MXD4 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer ...read more
Immunohistochemistry-Paraffin: MAD4 Antibody (OTI7F7) [NBP2-46060] - Paraffin-embedded human Kidney tissue within the normal limits using antiMXD4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA ...read more
Immunohistochemistry-Paraffin: MAD4 Antibody (OTI7F7) [NBP2-46060] - Human liver tissue using anti-MXD4 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 C for 3 ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
OTI7F7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

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MAD4 Antibody (OTI7F7) Summary

Immunogen
Full length human recombinant protein of human MXD4(NP_006445) produced in E.coli.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
MXD4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
Theoretical MW
23.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NBP2-46060 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MAD4 Antibody (OTI7F7)

  • BHLHC12
  • bHLHc12Max-interacting transcriptional repressor MAD4
  • Class C basic helix-loop-helix protein 12
  • Mad4 homolog
  • MAD4Max-associated protein 4
  • MAX dimerization protein 4
  • Max dimerizer 4
  • MST149
  • MSTP149
  • MXD4 MAX dimerization protein 4

Background

MAD4 is a member of the MAD gene family . The MAD genes encode basic helix loop helix leucine zipper proteins that heterodimerize with MAX protein, forming a transcriptional repression complex. The MAD proteins compete for MAX binding with MYC, which heterodimerizes with MAX forming a transcriptional activation complex. Studies in rodents suggest that the MAD genes are tumor suppressors and contribute to the regulation of cell growth in differentiating tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MAD4 Antibody (NBP2-46060) (0)

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Review for MAD4 Antibody (NBP2-46060) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-46060:
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ICC
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry MAD4 NBP2-46060
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4
reviewed by:
Julien COUTIER
ICC Human 08/30/2018
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Summary

ApplicationImmunocytochemistry
Sample TestedHuman keratinocytes
SpeciesHuman
LotW0001

Comments

CommentsPFA-fixed cells, permabilisation step is PBS-Triton 0.1% 20 minutes at room temperature, primary antibody used at 1/250e in PBS-BSA 2%, secondary antiboy conjugated to Alexa594

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MAD4 Antibody (NBP2-46060) (0)

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Secondary Antibodies

 

Isotype Controls

Additional MAD4 Products

Array NBP2-46060

Research Areas for MAD4 Antibody (NBP2-46060)

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Blogs on MAD4

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Julien COUTIER
08/30/2018
Application: ICC
Species: Human

Bioinformatics

Gene Symbol MXD4