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MAD4 Antibody (OTI7F7) - Azide and BSA Free

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Western Blot: MAD4 Antibody (OTI7F7) - Azide and BSA Free [NBP2-72567] - HEK293T cells were transfected with the pCMV6- ENTRY control (Left lane) or pCMV6-ENTRY MXD4 cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with antiMXD4.(1:2000)
Western Blot: MAD4 Antibody (OTI7F7) - Azide and BSA Free [NBP2-72567] - HEK293T cells were transfected with the pCMV6- ENTRY control (Left lane) or pCMV6-ENTRY MXD4 cDNA for 48 hrs and lysed. Equivalent amounts of cell ...read more
Immunohistochemistry: MAD4 Antibody (OTI7F7) - Azide and BSA Free [NBP2-72567] - Human tonsil within the normal limits using anti-MXD4 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris ...read more
Flow Cytometry: MAD4 Antibody (OTI7F7) - Azide and BSA Free [NBP2-72567] - Human keratinocytes, PFA-fixed, permeabilization: PBS-Triton 0.1% 20 minutes at RT, primary antibody at 1:250 in PBS-2% BSA, secondary antibody ...read more
Immunohistochemistry: MAD4 Antibody (OTI7F7) - Azide and BSA Free [NBP2-72567] - Human liver tissue using anti-MXD4 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at ...read more
Immunohistochemistry: MAD4 Antibody (OTI7F7) - Azide and BSA Free [NBP2-72567] - Paraffin-embedded human Kidney tissue within the normal limits using antiMXD4 mouse monoclonal antibody. (Heat-induced epitope retrieval ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, IHC
Clone
OTI7F7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-72567AF350
Alexa Fluor 405
NBP2-72567AF405
Alexa Fluor 488
NBP2-72567AF488
Alexa Fluor 532
NBP2-72567AF532
Alexa Fluor 594
NBP2-72567AF594
Alexa Fluor 647
NBP2-72567AF647
Alexa Fluor 700
NBP2-72567AF700
Alexa Fluor 750
NBP2-72567AF750
Allophycocyanin
NBP2-72567APC
Biotin
NBP2-72567B
CoraFluor 1
NBP2-72567CL1
DyLight 350
NBP2-72567UV
DyLight 405
NBP2-72567V
DyLight 488
NBP2-72567G
DyLight 550
NBP2-72567R
DyLight 594
NBP2-72567DL594
DyLight 650
NBP2-72567C
DyLight 680
NBP2-72567FR
DyLight 755
NBP2-72567IR
FITC
NBP2-72567F
HRP
NBP2-72567H
Janelia Fluor 525
NBP2-72567JF525
Janelia Fluor 549
NBP2-72567JF549
Janelia Fluor 585
NBP2-72567JF585
Janelia Fluor 635
NBP2-72567JF635
Janelia Fluor 646
NBP2-72567JF646
Janelia Fluor 669
NBP2-72567JF669
PE
NBP2-72567PE
PerCP
NBP2-72567PCP
Unconjugated
NBP2-46060
mFluor Violet 450 SE
NBP2-72567MFV450
mFluor Violet 500 SE
NBP2-72567MFV500
mFluor Violet 610 SE
NBP2-72567MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-46060
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MAD4 Antibody (OTI7F7) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human MXD4(NP_006445) produced in E.coli.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
MXD4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
Theoretical MW
23.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for MAD4 Antibody (OTI7F7) - Azide and BSA Free

  • BHLHC12
  • bHLHc12Max-interacting transcriptional repressor MAD4
  • Class C basic helix-loop-helix protein 12
  • Mad4 homolog
  • MAD4Max-associated protein 4
  • MAX dimerization protein 4
  • Max dimerizer 4
  • MST149
  • MSTP149
  • MXD4 MAX dimerization protein 4

Background

MAD4 is a member of the MAD gene family . The MAD genes encode basic helix loop helix leucine zipper proteins that heterodimerize with MAX protein, forming a transcriptional repression complex. The MAD proteins compete for MAX binding with MYC, which heterodimerizes with MAX forming a transcriptional activation complex. Studies in rodents suggest that the MAD genes are tumor suppressors and contribute to the regulation of cell growth in differentiating tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP2-72567
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Bioinformatics

Gene Symbol MXD4