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M-CSF Antibody

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Western Blot: M-CSF Antibody [NBP1-59425] - Sample Tissue: Human Fetal Liver Antibody Dilution: 1.0 ug/ml
Western Blot: M-CSF Antibody [NBP1-59425] - Sample Tissue: Human Fetal Liver Antibody Dilution: 1.0 ug/ml
Immunohistochemistry-Paraffin: M-CSF Antibody [NBP1-59425] - Human Placenta Tissue, 10 ug/ml
Western Blot: M-CSF Antibody [NBP1-59425] - WB Suggested Anti-CSF1 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:62500 Positive Control: COLO205 cell lysate
Western Blot: M-CSF Antibody [NBP1-59425] - Expressed in Human 721_B cells.
Western Blot: M-CSF Antibody [NBP1-59425] - Antibody Titration: 1 ug/ml Human heart.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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M-CSF Antibody Summary

Description
The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.
Immunogen
Synthetic peptides corresponding to MCSF The peptide sequence was selected from the middle region of MCSF. Peptide sequence SGSVLPLGELEGRRSTRDRRSPAEPEGGPASEGAARPLPRFNSVPLTDTG. The peptide sequence for this immunogen was taken from within the described region.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CSF1
Purity
Affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1.0 ug/ml
Theoretical MW
57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 2% Sucrose
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Affinity purified

Alternate Names for M-CSF Antibody

  • colony stimulating factor 1 (macrophage)
  • CSF1
  • CSF-1
  • Lanimostim
  • macrophage colony stimulating factor
  • macrophage colony-stimulating factor 1
  • MCSF
  • M-CSF
  • MCSFlanimostim
  • MGC31930

Background

Human Macrophage Colony Stimulating Factor (M-CSF, also called CSF-1) is a ~36 kDa disulfide-linked homodimer produced by a variety of cells including fibroblasts, macrophages, bone marrow stromal cells and endothelial cells. M-CSF regulates macrophage survival, proliferation and differentiation. M-CSF also enhances macrophage killing of tumor cells and microorganisms and regulates cytokine production from macrophages.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CSF1
Uniprot