Home » Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 » Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibodies » Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody [Janelia Fluor® 635]
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Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody [Janelia Fluor® 635]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Janelia Fluor 635

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-82107AF350
Alexa Fluor 405
NBP2-82107AF405
Alexa Fluor 488
NBP2-82107AF488
Alexa Fluor 532
NBP2-82107AF532
Alexa Fluor 594
NBP2-82107AF594
Alexa Fluor 647
NBP2-82107AF647
Alexa Fluor 700
NBP2-82107AF700
Alexa Fluor 750
NBP2-82107AF750
Biotin
NBP2-82107B
CoraFluor 1
NBP2-82107CL1
DyLight 350
NBP2-82107UV
DyLight 405
NBP2-82107V
DyLight 488
NBP2-82107G
DyLight 550
NBP2-82107R
DyLight 594
NBP2-82107DL594
DyLight 650
NBP2-82107C
DyLight 680
NBP2-82107FR
DyLight 755
NBP2-82107IR
FITC
NBP2-82107F
HRP
NBP2-82107H
Janelia Fluor 525
NBP2-82107JF525
Janelia Fluor 549
NBP2-82107JF549
Janelia Fluor 585
NBP2-82107JF585
Janelia Fluor 646
NBP2-82107JF646
Janelia Fluor 669
NBP2-82107JF669
PerCP
NBP2-82107PCP
Unconjugated
NBP2-82107
mFluor Violet 450 SE
NBP2-82107MFV450
mFluor Violet 500 SE
NBP2-82107MFV500
mFluor Violet 610 SE
NBP2-82107MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-82107
BSA Free
NBP2-82107SS
BSA Free
NBP2-82107
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Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody [Janelia Fluor® 635] Summary

Immunogen
Antibody was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3. The immunogen is located within amino acids 1370-1420 of Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3. Amino Acid Sequence: KVPGSRTPGHQENNNF
Specificity
This antibody is specific for Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 and will not recognize other JMJD proteins. Human Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 has 2 isoforms, including isoform 1 (1682aa, 181kD) and isoform 2 (1643aa, 177kD). Mouse Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 has one isoform (1641aa, 176kD) and rat Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 also has one isoform (1356aa, 146kD). NBP2-82107 can detect human, mouse and rat.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KDM6B
Purity
Peptide affinity purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Peptide affinity purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody [Janelia Fluor® 635]

  • EC 1.14.11
  • EC 1.14.11.-
  • JmjC domain-containing protein 3
  • JMJD3
  • JMJD3lysine-specific demethylase 6B
  • jumonji domain containing 3, histone lysine demethylase
  • Jumonji domain-containing protein 3
  • KDM6B
  • KIAA0346jumonji domain containing 3
  • Lysine (K)specific Demethylase 6B
  • Lysine (K)-specific Demethylase 6B
  • Lysine demethylase 6B

Background

JMJD3 is a histone demethylase that specifically demethylates trimethylated and dimethylated 'Lys-27' of histone H3, thereby playing a central role in histone code. JMJD3 is also important for the regulation of posterior development by regulating HOX gene expression. JMJD3 is involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation. It has also been shown to be essential to mammalian epidermal differentiation and is upregulated in prostate cancer.

JMJD3 antibodies are useful tools for epigenetics research and specifically for histone modification studies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody (NBP2-82107JF635) (0)

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Video Protocols

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ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol KDM6B