![Western Blot: LRP-1 Antibody (SA0290) [NBP2-67286] - Western blot analysis of LRP-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rab](http://images.novusbio.com/fullsize/LRP-1-Antibody-SA0290-Western-Blot-NBP2-67286-img0011.jpg "Western Blot: LRP-1 Antibody (SA0290) [NBP2-67286] - Western blot analysis of LRP-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rab")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, IP |
| Clone | SA0290 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Synthetic peptide within Human LRP-1 aa 4,471-4,520 / 4,544. (SwissProt: Q07954 Human; SwissProt: Q91ZX7 Mouse; SwissProt: G3V928 Rat) |
| Localization | Cytoplasm, Nucleus, Membrane. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | LRP1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | IP-assay-dependent |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
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Detecting HIF alpha and beyond: Best controls for hypoxia Western blot analysis By Rosa Moreno, PhD. Detecting HIF alpha and beyond: Best controls for hypoxia Western blot analysisPhysiological low levels of oxygen induce normal hypoxic events across biological systems. This hypoxic state activ... Read full blog post. |
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| Gene Symbol | LRP1 |
![Immunocytochemistry/Immunofluorescence: LRP-1 Antibody (SA0290) [NBP2-67286] - Staining LRP-1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/LRP-1-Antibody-SA0290-Immunocytochemistry-Immunofluorescence-NBP2-67286-img0004.jpg "Immunocytochemistry/Immunofluorescence: LRP-1 Antibody (SA0290) [NBP2-67286] - Staining LRP-1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: LRP-1 Antibody (SA0290) [NBP2-67286] - Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-LRP-1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30](http://images.novusbio.com/fullsize/LRP-1-Antibody-SA0290-Immunohistochemistry-Paraffin-NBP2-67286-img0010.jpg "Immunohistochemistry-Paraffin: LRP-1 Antibody (SA0290) [NBP2-67286] - Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-LRP-1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30")
![Flow Cytometry: LRP-1 Antibody (SA0290) [NBP2-67286] - Analysis of Hela cells with LRP1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/LRP-1-Antibody-SA0290-Flow-Cytometry-NBP2-67286-img0001.jpg "Flow Cytometry: LRP-1 Antibody (SA0290) [NBP2-67286] - Analysis of Hela cells with LRP1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.")
![Western Blot: LRP-1 Antibody (SA0290) [NBP2-67286] - Analysis of LRP-1 on different lysates using LRP-1 antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse liver Lane 2: Mouse brain Lane Lane 3: Mouse lung Lane 4: Human liver Lane 5: HepG2 Lane 6: Human lung](http://images.novusbio.com/fullsize/LRP-1-Antibody-SA0290-Western-Blot-NBP2-67286-img0009.jpg "Western Blot: LRP-1 Antibody (SA0290) [NBP2-67286] - Analysis of LRP-1 on different lysates using LRP-1 antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse liver Lane 2: Mouse brain Lane Lane 3: Mouse lung Lane 4: Human liver Lane 5: HepG2 Lane 6: Human lung")
![Immunocytochemistry/Immunofluorescence: LRP-1 Antibody (SA0290) [NBP2-67286] - Staining LRP-1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/LRP-1-Antibody-SA0290-Immunocytochemistry-Immunofluorescence-NBP2-67286-img0002.jpg "Immunocytochemistry/Immunofluorescence: LRP-1 Antibody (SA0290) [NBP2-67286] - Staining LRP-1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunocytochemistry/Immunofluorescence: LRP-1 Antibody (SA0290) [NBP2-67286] - Staining LRP-1 in HUVEC cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/LRP-1-Antibody-SA0290-Immunocytochemistry-Immunofluorescence-NBP2-67286-img0003.jpg "Immunocytochemistry/Immunofluorescence: LRP-1 Antibody (SA0290) [NBP2-67286] - Staining LRP-1 in HUVEC cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: LRP-1 Antibody (SA0290) [NBP2-67286] - Analysis of paraffin-embedded human liver tissue using LRP-1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/LRP-1-Antibody-SA0290-Immunohistochemistry-Paraffin-NBP2-67286-img0005.jpg "Immunohistochemistry-Paraffin: LRP-1 Antibody (SA0290) [NBP2-67286] - Analysis of paraffin-embedded human liver tissue using LRP-1 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: LRP-1 Antibody (SA0290) [NBP2-67286] - Analysis of paraffin-embedded human lung tissue using LRP-1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/LRP-1-Antibody-SA0290-Immunohistochemistry-Paraffin-NBP2-67286-img0006.jpg "Immunohistochemistry-Paraffin: LRP-1 Antibody (SA0290) [NBP2-67286] - Analysis of paraffin-embedded human lung tissue using LRP-1 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: LRP-1 Antibody (SA0290) [NBP2-67286] - Analysis of paraffin-embedded mouse brain tissue using LRP-1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/LRP-1-Antibody-SA0290-Immunohistochemistry-Paraffin-NBP2-67286-img0007.jpg "Immunohistochemistry-Paraffin: LRP-1 Antibody (SA0290) [NBP2-67286] - Analysis of paraffin-embedded mouse brain tissue using LRP-1 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: LRP-1 Antibody (SA0290) [NBP2-67286] - Analysis of paraffin-embedded mouse liver tissue using LRP-1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/LRP-1-Antibody-SA0290-Immunohistochemistry-Paraffin-NBP2-67286-img0008.jpg "Immunohistochemistry-Paraffin: LRP-1 Antibody (SA0290) [NBP2-67286] - Analysis of paraffin-embedded mouse liver tissue using LRP-1 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry LDLR Antibody [Unconjugated]](https://images.novusbio.com/images2/LDL_R_AF2255_Immunohistochemistry_7149.jpg)
![Western Blot LDLR Antibody [Unconjugated]](https://images.novusbio.com/images2/LDLR_AF2255_Western_Blot_23731.jpg)
![Western Blot LDLR Antibody [Unconjugated]](https://images.novusbio.com/images/af2255_mouse-ldl-r-affinity-purified-polyclonal-ab-4120241049445.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/DATA_IL6_M6000_ELISA_936.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/IL-6_M6000_ELISA_415.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images/m6000b_mouse-il-6-quantikine-elisa-kit-44202415433118.jpg)
![N/A t-Plasminogen Activator/tPA [HRP]](https://images.novusbio.com/images2/DATA_tPlasminogen_Activator_DTPA00_ELISA_854.jpg)
![N/A t-Plasminogen Activator/tPA [HRP]](https://images.novusbio.com/images2/tPlasminogen_Activator_DTPA00_ELISA_510.jpg)
![Enzyme Activity u-Plasminogen Activator/Urokinase [Unconjugated]](https://images.novusbio.com/images2/uPlasminogen_Activator_1310SE_1837.jpg)
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![Bioactivity CTLA-4 [Unconjugated]](https://images.novusbio.com/images2/CTLA4_7268CT_2293.jpg)
![Immunohistochemistry Lipoprotein Lipase/LPL Antibody [Unconjugated]](https://images.novusbio.com/images2/Lipoprotein_Lipase_AF7197_Immunohistochemistry_21186.jpg)
![Western Blot Lipoprotein Lipase/LPL Antibody [Unconjugated]](https://images.novusbio.com/images2/Lipoprotein_Lipase_AF7197_Western_Blot_11656.jpg)
![Immunocytochemistry Lipoprotein Lipase/LPL Antibody [Unconjugated]](https://images.novusbio.com/images2/Lipoprotein_Lipase_AF7197_Immunocytochemistry_11911.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")