LRH-1/NR5A2 Antibody Summary
Immunogen |
A portion of amino acids 300-350 from human Lrh-1 was used as the immunogen for this antibody. |
Predicted Species |
Orangutan (100%), Rabbit (94%), Chimpanzee (100%), Amphibian (94%), Sheep (94%), Monkey (100%). Backed by our 100% Guarantee. |
Isotype |
IgG |
Clonality |
Polyclonal |
Host |
Rabbit |
Gene |
NR5A2 |
Purity |
Protein A purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Simple Western 1:10
- Western Blot 2-4ug/ml
|
Application Notes |
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size. |
Reactivity Notes
The amino acid sequence used as immunogen for this Lrh-1 antibody is 100% homologous in White-cheeked Gibbon, Macaque, 88% in Porcine, and Bovine, and 83% in Zebrafish.
Packaging, Storage & Formulations
Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer |
0.2 ml PBS and 0.05% BSA |
Preservative |
0.05% Sodium Azide |
Concentration |
0.5 mg/ml |
Purity |
Protein A purified |
Alternate Names for LRH-1/NR5A2 Antibody
Background
Lrh-1 (Liver Receptor Homolog 1), also called NR5A2, appears to play an important role in regulating pancreatic and embryonal stem cell differentiation maintaining a pluripotential or undifferentiated state. It also plays a role in controlling cholesterol balance (1, 2). Lrh-1 may control pluripotentiality through its regulation of Oct4 and Nanog, both are commonly held as requisite factors for maintenance of stem cell undifferentiated states (3). Additionally, Lrh-1 has a role in colon, pancreatic and breast cancer, accompanied by overexpression in the latter in ductal carcinomas (4, 5). Antibody to Lrh-1 provides a tool to link expression and function with undifferentiated states in embryonic stem cells or in cancers of the breast, intestine, and pancreas, and for further characterization of Lrh-1 as a potential biomarker.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Secondary Antibodies
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