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LRH-1/NR5A2 Antibody

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Western Blot: LRH-1/NR5A2 Antibody [NBP2-27204] - Analysis of Lrh-1. HEPG2 lysate in the 1) absence and 2) presence of immunizing peptide, 3) RAW lysate probed with 2 ug/ml of Lrh-1 antibody. Goat anti-rabbit Ig HRP ...read more
Simple Western: LRH-1/NR5A2 Antibody [NBP2-27204] - Simple Western lane view shows a specific band for LRH-1/NR5A2 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 ...read more

Product Details

Summary
Reactivity Hu, Ca, Ch, Eq, Fe, Gp, Ma-Op, Pm, Am, Pm, Pm, Pm-Or, Rb, ShSpecies Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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LRH-1/NR5A2 Antibody Summary

Immunogen
A portion of amino acids 300-350 from human Lrh-1 was used as the immunogen for this antibody.
Predicted Species
Orangutan (100%), Rabbit (94%), Chimpanzee (100%), Amphibian (94%), Sheep (94%), Monkey (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NR5A2
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Simple Western 1:10
  • Western Blot 2-4ug/ml
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.

Reactivity Notes

The amino acid sequence used as immunogen for this Lrh-1 antibody is 100% homologous in White-cheeked Gibbon, Macaque, 88% in Porcine, and Bovine, and 83% in Zebrafish.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.2 ml PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A purified

Alternate Names for LRH-1/NR5A2 Antibody

  • Alpha-1-fetoprotein transcription factor
  • B1-binding factor
  • b1-binding factor, hepatocyte transcription factor which activates enhancer IIof hepatitis B virus
  • B1F
  • B1F2
  • fetoprotein-alpha 1 (AFP) transcription factor
  • FTFLRH1
  • FTZ-F1 beta
  • FTZ-F1
  • FTZ-F1beta
  • HB1F
  • hB1F-2
  • hB1FCYP7A promoter-binding factor
  • Hepatocytic transcription factor
  • Liver receptor homolog 1
  • liver receptor homolog-1
  • LRH1
  • LRH-1
  • LRH-1CPF
  • NR5A2
  • nuclear receptor NR5A2
  • nuclear receptor subfamily 5 group A member 2
  • nuclear receptor subfamily 5, group A, member 2

Background

Lrh-1 (Liver Receptor Homolog 1), also called NR5A2, appears to play an important role in regulating pancreatic and embryonal stem cell differentiation maintaining a pluripotential or undifferentiated state. It also plays a role in controlling cholesterol balance (1, 2). Lrh-1 may control pluripotentiality through its regulation of Oct4 and Nanog, both are commonly held as requisite factors for maintenance of stem cell undifferentiated states (3). Additionally, Lrh-1 has a role in colon, pancreatic and breast cancer, accompanied by overexpression in the latter in ductal carcinomas (4, 5). Antibody to Lrh-1 provides a tool to link expression and function with undifferentiated states in embryonic stem cells or in cancers of the breast, intestine, and pancreas, and for further characterization of Lrh-1 as a potential biomarker.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol NR5A2