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Lipocalin-2/NGAL Antibody

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Western Blot: Lipocalin-2/NGAL Antibody [NB100-1503] - (0.03ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: Lipocalin-2/NGAL Antibody [NB100-1503] - (0.03ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Lipocalin-2/NGAL Antibody [NB100-1503] - Analysis of LCN2 in human xenograft tumor. Image courtesy of product review submitted by Yi-Ling Huang.
Immunohistochemistry-Paraffin: Lipocalin-2/NGAL Antibody [NB100-1503] - (2.5ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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Lipocalin-2/NGAL Antibody Summary

Immunogen
Peptide with sequence C-AILREDKDPQKMY corresponding to internal region according to NP_005555.2.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
LCN2
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 2 - 3 ug/ml
  • Immunohistochemistry-Paraffin 2 - 3 ug/ml
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.01 - 0.03 ug/ml
Application Notes
WB: Approx. 22 kDa band observed in human kidney lysates (calculated MW of 22.6 kDa band according to NP_005555.2). IHC-P: Human spleen shows staining of cytoplasm in mostly binucleated cells, and of nucleoli in some of the non-dividing cells.
Control
Lipocalin-2/NGAL Overexpression Lysate
Publications
Read Publications using
NB100-1503 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Lipocalin-2/NGAL Antibody

  • 24p3
  • 25 kDa alpha-2-microglobulin-related subunit of MMP-9
  • HNL
  • LCN2
  • lipocalin 2 (oncogene 24p3)
  • lipocalin 2
  • Lipocalin2
  • Lipocalin-2
  • migration-stimulating factor inhibitor
  • MSFI
  • neutrophil gelatinase-associated lipocalin
  • NGAL
  • NGALlipocalin-2
  • Oncogene 24p3
  • p25
  • Siderocalin
  • Uterocalin

Background

Lipocalin 2 is a member of the lipocalin family which encompass more than 25 members (including proteins like retinol binding protein, a1 microglobulin, b lactoglobulin, apolipoprotein D, odorant binding protein). The members of the lipocalin family are characterized by their ability to bind small lipophilic substances in their hydrophobic core, and thereby serve as transporters of substances.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Lipocalin-2/NGAL
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NB100-1503
Lipocalin-2/NGAL Antibody
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Publications for Lipocalin-2/NGAL Antibody (NB100-1503)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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(1)
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Video Protocols

WB Video Protocol

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Control Lysate(s)

Lipocalin-2/NGAL Overexpression Lysate

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol LCN2
Entrez
Uniprot