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Lipocalin-2/NGAL Antibody (29) - BSA Free

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Immunohistochemistry: Lipocalin-2/NGAL Antibody (29) - BSA Free [NBP1-05183] - NGAL expression 2 days after the introduction of ischemic-reperfusion injured mouse kidney;detected with anti-mouse NGAL antibody at ...read more
Immunohistochemistry: Lipocalin-2/NGAL Antibody (29) - BSA Free [NBP1-05183] - NGAL expression in the ischemia-reperfusion injured mouse kidney; detected with anti-mouse NGAL antibody at dilution of 1:1000. Image and ...read more
ELISA: Lipocalin-2/NGAL Antibody (29) - BSA Free [NBP1-05183] - The figure illustrates the calibration curve of a sandwich assay with NBP2-21746 as the capture antibody and NBP1-05183B as the biotinylated detection ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA, IHC
Clone
29
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

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Lipocalin-2/NGAL Antibody (29) - BSA Free Summary

Immunogen
Recombinant mouse NGAL
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Rat
Gene
LCN2
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • ELISA 1:4000
  • Immunohistochemistry 1:1000
  • Immunohistochemistry-Paraffin 1:1000
  • Western Blot 1:100-1:2000
Application Notes
ELISA: NBP1-05183 binds free mouse NGAL in solution. NBP1-05183 forms an antibody pair for sandwich ELISA with NBP2-21746. It is recommended to use NBP2-21746 as a capture antibody and biotinylated NBP1-05183 as a detection antibody. WB: NBP1-05183 can be used in Western blotting. IHC: NBP1-05183 detects NGAL in paraffin-embedded sections in ischemia-reperfusion injured kidney cells from mouse (dilution 1:1000). Microwave pretreatment using Citrat buffer pH 6, is recommended.
Publications
Read Publications using
NBP1-05183 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
10mM Phosphate (pH 7.4) and 0.5M NaCl
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Alternate Names for Lipocalin-2/NGAL Antibody (29) - BSA Free

  • 24p3
  • 25 kDa alpha-2-microglobulin-related subunit of MMP-9
  • HNL
  • LCN2
  • lipocalin 2 (oncogene 24p3)
  • lipocalin 2
  • Lipocalin2
  • Lipocalin-2
  • migration-stimulating factor inhibitor
  • MSFI
  • neutrophil gelatinase-associated lipocalin
  • NGAL
  • NGALlipocalin-2
  • Oncogene 24p3
  • p25
  • Siderocalin
  • Uterocalin

Background

Mouse neutrophil gelatinase-associated lipocalin (NGAL; also called lipocalin 2, 24p3, SIP24, uterocalin and siderocalin) is a member of the lipocalin family of proteins which bind and transport small lipophilic molecules. 24p3 was observed to be dramatically upregulated in kidney cell cultures infected with SV40 (1) and subsequently identified as a lipocalin (2). Unlike human NGAL, it is an acute phase protein upregulated in the liver (3), but like its mammalian homologues it is upregulated and secreted by the kidney in response to ischemic or nephrotoxic injury (4). It is also upregulated in the involuting uterus and mammary gland (5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Lipocalin-2/NGAL Antibody (NBP1-05183)(2)

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Bioinformatics

Gene Symbol LCN2