Home » Lipocalin-2/NGAL » Lipocalin-2/NGAL Antibodies » Lipocalin-2/NGAL Antibody (228421)
Novus Biologicals products are now on bio-techne.com

Lipocalin-2/NGAL Antibody (228421)

 (3 Publications)
 See All Related
Submit a Review

Images

 
(
Enlarge)
Epitope mapping of LCN2 mAb. (A) ELISA quantification of relative binding of LCN2 mAb to 56 peptides covering the mouse LCN2 protein (n = 3); (B) The combined sequences of peptides 22–24 are shown in red at amino acids 64–84 of the mouse LCN2 protein. The N-terminal signal peptide that was cleaved prior to the secretion of the protein is underlined and not included in the epitope mapping. Cys-78 and Cys-177 forming an intramolecular disulfide bond (blue); (C) Alignment of the LCN2 mAb epitope sequences from mouse, rat, and human LCN2 homologs. Conserved amino acids are underlined. Amino acids interacting with siderophore are highlighted in pink. Conserved cysteines are highlighted in blue; (D) The crystal structure of human LCN2 protein containing a siderophore (black) and an iron (orange). The epitope of LCN2 mAb, highlighted in red, is located in the beta 3 and beta 4 strands. The side chains of amino acids bound with siderophore are colored in pink. An intramolecular disulfide bond formed between Cys-78 in the epitope and Cys-177 near the C-terminal (blue); (E) Representative Western blot showing that increased concentrations of epitope peptides from mouse (YNVTSILVRDQDQGCRYWIRT) and human (YNVTSVLFRKKKCDYWIRT) tissue reduced the level of immunoprecipitated mouse (mLCN2) or human LCN2 protein (hLCN2). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32872405), licensed under a CC-BY license. Not internally tested by R&D Systems.
Epitope mapping of LCN2 mAb. (A) ELISA quantification of relative binding of LCN2 mAb to 56 peptides covering the mouse LCN2 protein (n = 3); (B) The combined sequences of peptides 22–24 are shown in red at amino ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA(Cap)
Clone
228421
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB18571-SP
Carrier Free
MAB18571-500
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

Lipocalin-2/NGAL Antibody (228421) Summary

Immunogen
NS0-derived recombinant mouse Lipocalin-2/NGAL
Gln21-Asn200
Accession # P11672
Specificity
Detects mouse Lipocalin-2/NGAL in ELISAs. In sandwich immunoassays, no cross-reactivity with recombinant human (rh) Lipocalin-1, rhLipocalin-2 or recombinant rat Lipocalin-2 is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
LCN2
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.5-2.0 ug/mL
  • ELISA Standard (Matched Pair)
Application Notes
ELISA Capture: Mouse Lipocalin-2/NGAL Antibody (Catalog # MAB18571)
ELISA Detection: Mouse Lipocalin-2/NGAL Biotinylated Antibody (Catalog # BAM1857)
Standard: Recombinant Mouse Lipocalin-2/NGAL (Catalog # 1857-LC)
Publications
Read Publications using
MAB18571 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Lipocalin-2/NGAL Antibody (228421)

  • 24p3
  • 25 kDa alpha-2-microglobulin-related subunit of MMP-9
  • HNL
  • LCN2
  • lipocalin 2 (oncogene 24p3)
  • lipocalin 2
  • Lipocalin2
  • Lipocalin-2
  • migration-stimulating factor inhibitor
  • MSFI
  • neutrophil gelatinase-associated lipocalin
  • NGAL
  • NGALlipocalin-2
  • Oncogene 24p3
  • p25
  • Siderocalin
  • Uterocalin

Background

Mouse Lipocalin-2 was cloned from mouse kidney cells (1). Its very high level of expression at the post-stratum uterus gave it the name uterocalin (2). Lipocalin-2 has been implicated in a variety of processes including cell differentiation, tumorigenesis, and apoptosis (3‑5). Studies indicate that Lipocalin-2 binds a bacterial catecholate siderophore that is bound to a ferric ion, such as enterobactin, with a subnanomolar dissociation constant (KD = 0.41 nM) (6). The bound ferric enterobactin complex breaks down slowly in a month into dihydroxybenzoyl serine and dihydroxybenzoic acid (DHBA). It also binds to a ferric DHBA complex with much less KD values (7.9 nM) (6). Secretion of Lipocalin-2 in immune cells increases in response to stimulation of Toll-like receptor as an acute phase response to infection. As a result, it acts as a potent bacteriostatic reagent by sequestering iron (7). Moreover, Lipocalin-2 can alter the invasive and metastatic behavior of Ras-transformed breast cancer cells in vitro and in vivo by reversing the epithelial to mesenchymal transition inducing activity of Ras, through restoration of E-cadherin expression, via effects on the Ras-MAPK signaling pathway (8).

  1. Hraba-Renevey, s. et al. (1989) Oncogene. 4:601. 
  2. Liu, Q. et al. (1993) Mol Reprod Dev. 46:507. 
  3. Kjeldsen L, et al. (2000) Biochim Biophys Acta. 1482:272.
  4. Devireddy, L.R. et al. (2001) Science 293:829.
  5. Yang, M.B. et al. (2002) Mol. Cell. 10:1045.
  6. Goetz, D.H. et al. (2002) Mol. Cell 10:1033.
  7. Flo, T.H. et al. (2004) Nature 432:917.
  8. Hanai, J. et al. (2005) J. Biol. Chem. 280:13641.
 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Cdk5 Antibody
NBP2-15843
Western Blot Cdk5 Antibody
Immunohistochemistry-Paraffin Cdk5 Antibody
Immunocytochemistry/ Immunofluorescence Cdk5 Antibody
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KO, WB
TIM-1/KIM-1/HAVCR
Antibody [Unconjugate ...
AF1817
Western Blot TIM-1/KIM-1/HAVCR Antibody [Unconjugated]
Western Blot TIM-1/KIM-1/HAVCR Antibody [Unconjugated]
(105 Publications)
Species: Mu
Applications: WB
Cystatin C [HRP]
MSCTC0
N/A Cystatin C [HRP]
N/A Cystatin C [HRP]
N/A Cystatin C [HRP]
(37 Publications)
Species: Mu, Rt
Applications: ELISA
IL-18/IL-1F4 [HRP]
7625
IL-18/IL-1F4 [HRP]
(54 Publications)
Species: Mu
Applications: ELISA
MMP-9 [HRP]
DMP900
N/A MMP-9 [HRP]
N/A MMP-9 [HRP]
(220 Publications)
Species: Hu
Applications: ELISA
CDK5 Activator 1
Antibody
NBP3-13279
Immunocytochemistry/ Immunofluorescence CDK5 Activator 1 Antibody
Immunohistochemistry-Paraffin CDK5 Activator 1 Antibody
Immunohistochemistry-Paraffin CDK5 Activator 1 Antibody
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(818 Publications)
Species: Hu
Applications: BA
CDCA5 Antibody
NBP1-89530
Western Blot CDCA5 Antibody
Immunohistochemistry-Paraffin CDCA5 Antibody
Immunocytochemistry/ Immunofluorescence CDCA5 Antibody
(1 Publication)
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
Ficolin-2
[Unconjugated]
2428-FC
Ficolin-2 [Unconjugated]
(2 Publications)
Species: Hu
Applications: Bind
Annexin A1
Antibody [Unconjugate
AF3770
Western Blot Annexin A1 Antibody [Unconjugated]
Immunohistochemistry Annexin A1 Antibody [Unconjugated]
Simple Western Annexin A1 Antibody [Unconjugated]
(1 Review)
(6 Publications)
Species: Hu, Mu, Rt
Applications: IHC, Simple Western, WB
Uroplakin IIIB
Antibody
NBP1-92564
Immunohistochemistry-Paraffin Uroplakin IIIB Antibody
Immunohistochemistry-Paraffin Uroplakin IIIB Antibody
Immunohistochemistry-Paraffin Uroplakin IIIB Antibody
(1 Review)
Species: Hu
Applications: ICC/IF, IHC, IHC-P
GCHFR Antibody
(846011) [Unconjugate
MAB7918
Simple Western GCHFR Antibody (846011) [Unconjugated]
Western Blot GCHFR Antibody (846011) [Unconjugated]
Species: Hu
Applications: Simple Western, WB
IL-12
[Unconjugated]
419-ML
SDS-PAGE IL-12 [Unconjugated]
Bioactivity IL-12 [Unconjugated]
(273 Publications)
Species: Mu
Applications: BA
IL-6 [HRP]
M6000B
N/A IL-6 [HRP]
N/A IL-6 [HRP]
N/A IL-6 [HRP]
(667 Publications)
Species: Mu
Applications: ELISA

Myeloperoxidase/MPO An ...
AF3667
Western Blot Myeloperoxidase/MPO Antibody [Unconjugated]
Simple Western Myeloperoxidase/MPO Antibody [Unconjugated]
Immunocytochemistry Myeloperoxidase/MPO Antibody [Unconjugated]
(3 Reviews)
(167 Publications)
Species: Hu, Mu
Applications: Dual ISH-IHC, ICC, IHC, Simple Western, WB
C-Reactive
Protein/CRP [Biotin]
DY1707
N/A C-Reactive Protein/CRP [Biotin]
(70 Publications)
Species: Hu
Applications: ELISA
MMP-2 [HRP]
MMP200
N/A MMP-2 [HRP]
N/A MMP-2 [HRP]
(102 Publications)
Species: Ca, Hu, Mu, Po, Rt
Applications: ELISA
Tau Antibody (2E9)
NBP2-25162
Immunocytochemistry/ Immunofluorescence Tau Antibody (2E9)
Western Blot Tau Antibody (2E9)
Immunocytochemistry/ Immunofluorescence Tau Antibody (2E9)
(5 Publications)
Species: Bv, Eq, Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, PLA, WB
TPPP/p25 Antibody
NBP2-34031
Western Blot TPPP/p25 Antibody
Immunohistochemistry-Paraffin TPPP/p25 Antibody
Immunohistochemistry-Paraffin TPPP/p25 Antibody
(1 Publication)
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
Lipocalin-2/NGAL
Antibody (228421)
MAB18571
Lipocalin-2/NGAL Antibody (228421)
Species: Mu
Applications: ELISA(Cap)

Publications for Lipocalin-2/NGAL Antibody (MAB18571)(3)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: Bioassay, Western Blot.


Filter By Application
Bioassay
(1)
Western Blot
(1)
All Applications
Filter By Species
Mouse
(2)
All Species

Reviews for Lipocalin-2/NGAL Antibody (MAB18571) (0)

There are no reviews for Lipocalin-2/NGAL Antibody (MAB18571). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for Lipocalin-2/NGAL Antibody (MAB18571) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Lipocalin-2/NGAL Products

Blogs on Lipocalin-2/NGAL

There are no specific blogs for Lipocalin-2/NGAL, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Lipocalin-2/NGAL Antibody (228421) and receive a gift card or discount.


Bioinformatics

Gene Symbol LCN2
Uniprot