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LIF Antibody [Unconjugated]

 (12 Publications)
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Recombinant Mouse LIF (Catalog # 8878-LF) stimulates IL-6 secretion in the M1 mouse myeloid leukemia cell line in a dose-dependent manner (orange line) as measured by the Mouse IL-6 Quantikine ELISA (Catalog # M6000B). IL-6 secretion elicited by 3 ng/mL Recombinant Mouse LIF is neutralized (green line) by increasing concentrations of Goat Anti-Mouse LIF Polyclonal Antibody (Catalog # AB-449-NA). The ND50 is typically 80-480 ng/mL.
Recombinant Mouse LIF (Catalog # 8878-LF) stimulates IL-6 secretion in the M1 mouse myeloid leukemia cell line in a dose-dependent manner (orange line) as measured by the Mouse IL-6 Quantikine ELISA (Catalog # M6000B). ...read more
LIF was detected in perfusion fixed frozen sections of mouse trigeminal ganglion using Goat Anti-Mouse LIF Polyclonal Antibody (Catalog # AB-449-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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LIF Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse LIF
Specificity
Detects mouse LIF in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human LIF is observed. Neutralizes the biological activity of recombinant mouse LIF and will also neutralize the biological activity of recombinant human LIF at a 10-fold higher IgG concentration.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
LIF
Purity Statement
Protein A or G purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 80-480 ng/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AB-449-NA in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 1 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LIF Antibody [Unconjugated]

  • CDF
  • D Factor
  • DIA
  • differentiation inhibitory activity
  • differentiation stimulating factor
  • Differentiation-stimulating factor
  • Emfilermin
  • HILDA
  • HILDAcholinergic differentiation factor
  • leukemia inhibitory factor (cholinergic differentiation factor)
  • leukemia inhibitory factor
  • LIF
  • Melanoma-derived LPL inhibitor
  • MLPLI

Background

Leukemia inhibitory factor (LIF) was initially identified as a factor that inhibited the proliferation and induced the differentiation to macrophages of the murine myeloid leukemic cell line M1. Subsequent to its purification and molecular cloning, LIF was recognized to be a pleiotropic factor with multiple effects on both hematopoietic and non-hematopoietic cells. LIF has overlapping biological functions with OSM, IL-6, IL-11 and CNTF. All these cytokines utilize gp130 as a component in their signal transducing receptor complexes. Mouse LIF cDNA encodes a 203 amino acid residue polypeptide with a 23 amino acid signal peptide that is cleaved to yield a 180 amino acid mature mouse LIF. Native human and mouse LIF are highly glycosylated monomeric proteins. Both human and murine LIF protein sequences have multiple potential N- and O-linked glycosylation sites and six conserved cysteine residues that are involved in three intramolecular disulfide bridges.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for LIF Antibody (AB-449-NA)(12)

We have publications tested in 2 confirmed species: Mouse, Transgenic Mouse.

We have publications tested in 6 applications: IHC, In Vivo, In vivo assay, Neutralization, Reference Standard, Western Blot.


Filter By Application
IHC
(4)
In Vivo
(1)
In vivo assay
(1)
Neutralization
(2)
Reference Standard
(1)
Western Blot
(3)
All Applications
Filter By Species
Mouse
(9)
Transgenic Mouse
(2)
All Species
Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using AB-449-NA Applications Species
Kakae, M;Nakajima, H;Tobori, S;Kawashita, A;Miyanohara, J;Morishima, M;Nagayasu, K;Nakagawa, T;Shigetomi, E;Koizumi, S;Mori, Y;Kaneko, S;Shirakawa, H; The astrocytic TRPA1 channel mediates an intrinsic protective response to vascular cognitive impairment via LIF production Science advances 2023-07-21 [PMID: 37478173] (In vivo assay, Neutralization, Mouse, Transgenic Mouse) In vivo assay, Neutralization Mouse, Transgenic Mouse
S Xu, X Yang, Q Chen, Z Liu, Y Chen, X Yao, A Xiao, J Tian, L Xie, M Zhou, Z Hu, F Zhu, X Xu, F Hou, J Nie Leukemia inhibitory factor is a therapeutic target for renal interstitial fibrosis EBioMedicine, 2022-11-04;86(0):104312. 2022-11-04 [PMID: 36335669] (IHC, In Vivo, Mouse) IHC, In Vivo Mouse
Wei Cai, Ligen Shi, Jingyan Zhao, Fei Xu, Connor Dufort, Qing Ye et al. Neuroprotection against ischemic stroke requires a specific class of early responder T cells in mice Journal of Clinical Investigation 8/1/2022 [PMID: 35912857]
JW Lee, J Hur, YW Kwon, CW Chae, JI Choi, I Hwang, JY Yun, JA Kang, YE Choi, YH Kim, SE Lee, C Lee, DH Jo, H Seok, BS Cho, SH Baek, HS Kim KAI1(CD82) is a key molecule to control angiogenesis and switch angiogenic milieu to quiescent state Journal of hematology & oncology, 2021-09-16;14(1):148. 2021-09-16 [PMID: 34530889] (Western Blot, Mouse) Western Blot Mouse
Long Ma, Xin Zhao, Yibin Liu, Jiang Wu, Xiaochun Yang, Qunhua Jin Dihydroartemisinin attenuates osteoarthritis by inhibiting abnormal bone remodeling and angiogenesis in subchondral bone International Journal of Molecular Medicine 1/15/2021 [PMID: 33448319]
Jingke Du, Jiancheng Yang, Zihao He, Junqi Cui, Yiqi Yang, Mingming Xu et al. Osteoblast and Osteoclast Activity Affect Bone Remodeling Upon Regulation by Mechanical Loading-Induced Leukemia Inhibitory Factor Expression in Osteocytes Frontiers in Molecular Biosciences 11/26/2020 [PMID: 33324677]
DA Skelly, A Czechanski, C Byers, S Aydin, C Spruce, C Olivier, K Choi, DM Gatti, N Raghupathy, GR Keele, A Stanton, M Vincent, S Dion, I Greenstein, M Pankratz, DK Porter, W Martin, C O'Connor, W Qin, AH Harrill, T Choi, GA Churchill, SC Munger, CL Baker, LG Reinholdt Mapping the Effects of Genetic Variation on Chromatin State and Gene Expression Reveals Loci That Control Ground State Pluripotency Cell Stem Cell, 2020-08-13;0(0):. 2020-08-13 [PMID: 32795400] (Neutralization, Reference Standard, Mouse) Neutralization, Reference Standard Mouse
M Koide, T Yamashita, K Murakami, S Uehara, K Nakamura, M Nakamura, M Matsushita, T Ara, H Yasuda, JM Penninger, N Takahashi, N Udagawa, Y Kobayashi Sclerostin expression in trabecular bone is downregulated by osteoclasts Sci Rep, 2020-08-13;10(1):13751. 2020-08-13 [PMID: 32792620] (IHC, Mouse) IHC Mouse
SS Welc, I Flores, M Wehling-He, J Ramos, Y Wang, C Bertoni, JG Tidball Targeting a therapeutic LIF transgene to muscle via the immune system ameliorates muscular dystrophy Nat Commun, 2019-06-26;10(1):2788. 2019-06-26 [PMID: 31243277] (IHC, Mouse, Transgenic Mouse) IHC Mouse, Transgenic Mouse
GK Arora, A Gupta, S Narayanan, T Guo, P Iyengar, RE Infante Cachexia-associated adipose loss induced by tumor-secreted leukemia inhibitory factor is counterbalanced by decreased leptin JCI Insight, 2018-07-26;3(14):. 2018-07-26 [PMID: 30046014] (Western Blot, Mouse) Western Blot Mouse
Show All 12 Publications.

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Bioinformatics

Gene Symbol LIF