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Laminin alpha 2 Antibody (CL3450)

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Orthogonal Strategies: Immunohistochemistry-Paraffin: Laminin alpha 2 Antibody (CL3450) [NBP2-46624] - Laminin alpha 2 Antibody (3450) [NBP2-46624] - Staining in human heart muscle and tonsil tissues. ...read more
Western Blot: Laminin alpha 2 Antibody (3450) [NBP2-46624] - Analysis of purified human recombinant Laminin-211, Laminin-221, Laminin-332, Laminin-421, Laminin-511 and Laminin-121.
Immunohistochemistry-Paraffin: Laminin alpha 2 Antibody (3450) [NBP2-46624] - Staining of mouse heart muscle shows strong membranous positivity in cardiomyocytes.
Immunohistochemistry-Paraffin: Laminin alpha 2 Antibody (3450) [NBP2-46624] - Staining of rat stomach shows strong positivity in basement membrane of glandular cells.
Immunohistochemistry-Paraffin: Laminin alpha 2 Antibody (3450) [NBP2-46624] - Staining of rat heart muscle shows strong membranous positivity in cardiomyocytes.
Immunohistochemistry-Paraffin: Laminin alpha 2 Antibody (3450) [NBP2-46624] - Staining of human heart muscle shows strong membranous positivity in cardiomyocytes.
Immunohistochemistry-Paraffin: Laminin alpha 2 Antibody (3450) [NBP2-46624] - Staining of human placenta shows moderate positivity in basement membrane of trophoblastic cells.
Immunohistochemistry-Paraffin: Laminin alpha 2 Antibody (3450) [NBP2-46624] - Staining of human cerebral cortex shows moderate positivity in blood vessels.
Immunohistochemistry-Paraffin: Laminin alpha 2 Antibody (3450) [NBP2-46624] - Staining of human tonsil shows no positivity in lymphoid cells as expected.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
CL3450
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

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Laminin alpha 2 Antibody (CL3450) Summary

Immunogen
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence DKLKPIKELEDNLKKNISEIKELINQARKQANSIKVSVSSGGDCIRTYKPEIKKGSYNNIVVNVKTAVADNLLFYLGSAKFIDFLAIEMRKGKVSFLWDVGSGVGRVEYPDLTIDDSYWYRIVASRTGRNGT
Epitope
DKLKPIKELEDNLKK
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
LAMA2
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200-1:500
  • Western Blot 1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
Laminin alpha 2 Recombinant Protein Antigen (NBP2-46624PEP)

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for Laminin alpha 2 Antibody (CL3450)

  • LAMA2
  • Laminin alpha 2
  • Laminin M chain
  • laminin subunit alpha-2
  • laminin, alpha 2
  • Laminin-12 subunit alpha
  • Laminin-2 subunit alpha
  • LAMM
  • LAMMLaminin-4 subunit alpha
  • Merosin heavy chain
  • Merosin

Background

Laminin, an extracellular protein, is a major component of the basement membrane. It is thought to mediate the attachment, migration, and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. It is composed of three subunits, alpha, beta, and gamma, which are bound to each other by disulfide bonds into a cross-shaped molecule. This gene encodes the alpha 2 chain, which constitutes one of the subunits of laminin 2 (merosin) and laminin 4 (s-merosin). Mutations in this gene have been identified as the cause of congenital merosin-deficient muscular dystrophy. Two transcript variants encoding different proteins have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol LAMA2
Entrez
Uniprot