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Laminin alpha 2 Antibody (1003531) [Unconjugated]

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Laminin alpha 2 was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human Laminin alpha 2 Monoclonal Antibody (Catalog # MAB2143) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes in cardiomyocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Laminin alpha 2 was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human Laminin alpha 2 Monoclonal Antibody (Catalog # MAB2143) at 5 µg/mL for 1 hour at room temperature ...read more
Laminin alpha 2 was detected in immersion fixed U2OS human osteosarcoma cell line (positive stain) and MCF-7 human breast cancer cell line (negative stain) using Mouse Anti-Human Laminin alpha 2 Monoclonal Antibody ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC, ICC/IF
Clone
1003531
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB2143-SP
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Laminin alpha 2 Antibody (1003531) [Unconjugated] Summary

Immunogen
Chinese Hamster Ovary cell line, CHO-derived human Laminin alpha 2
Lys2145-Asn3122
Accession # P24043
Specificity
Detects human Laminin alpha 2 in direct ELISAs.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 5-25 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Laminin alpha 2 Antibody (1003531) [Unconjugated]

  • LAMA2
  • Laminin alpha 2
  • Laminin M chain
  • laminin subunit alpha-2
  • laminin, alpha 2
  • Laminin-12 subunit alpha
  • Laminin-2 subunit alpha
  • LAMM
  • LAMMLaminin-4 subunit alpha
  • Merosin heavy chain
  • Merosin

Background

Laminin subunit alpha-2 (LAMA-2) is an extracellular matrix protein that is a main component of the basement membrane. It is thought to mediate the attachment, migration and organization of cells during embryonic development via interacting with other components with the extracellular matrix. It is widely expressed except in the liver, thymus and bone.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Laminin alpha 2
Antibody (1003531) [U ...
MAB2143
Laminin alpha 2 Antibody (1003531) [Unconjugated]
Species: Hu
Applications: IHC, ICC/IF

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