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LAG-3 Antibody (874528) [Unconjugated]

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HEK293 human embryonic kidney cell line transfected with either (A) human LAG-3 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human LAG-3 Monoclonal Antibody (Catalog # MAB23197) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041, data not shown). View our protocol for Staining Membrane-associated Proteins.
HEK293 human embryonic kidney cell line transfected with either (A) human LAG-3 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human LAG-3 Monoclonal Antibody (Catalog # MAB23197) followed by ...read more
LAG‑3 was detected in immersion fixed paraffin-embedded sections of mouse spleen using Mouse Anti-Human LAG‑3 Monoclonal Antibody (Catalog # MAB23197) at 15 µg/mL for 1 hour at room temperature ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IHC, CyTOF-ready
Clone
874528
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB23197U-100UG
Alexa Fluor 405
FAB23197V-100UG
Alexa Fluor 488
FAB23197G-100UG
Alexa Fluor 532
FAB23197X
Alexa Fluor 594
FAB23197T-100UG
Alexa Fluor 647
FAB23197R-100UG
Alexa Fluor 700
FAB23197N-100UG
Alexa Fluor 750
FAB23197S-100UG
Allophycocyanin
FAB23197A
Allophycocyanin/Cy7
FAB23197APCCY7
Biotin
FAB23197B
CoraFluor 1
FAB23197CL1
DyLight 350
FAB23197D
DyLight 405
FAB23197E
DyLight 488
FAB23197K
DyLight 550
FAB23197L
DyLight 594
FAB23197M
DyLight 650
FAB23197W
DyLight 680
FAB23197Y
DyLight 755
FAB23197Z
FITC
FAB23197F
HRP
FAB23197H
Janelia Fluor 525
FAB23197JF525
Janelia Fluor 549
FAB23197I
Janelia Fluor 585
FAB23197JF585
Janelia Fluor 635
FAB23197JF635
Janelia Fluor 646
FAB23197J
Janelia Fluor 669
FAB23197JF669
PE
FAB23197P
PE/Atto594
FAB23197PEATT594
PE/Cy5.5
FAB23197PECY55
PE/Cy7
FAB23197PECY7
PerCP
FAB23197C
mFluor Violet 450 SE
FAB23197MFV450
mFluor Violet 500 SE
FAB23197MFV500
mFluor Violet 610 SE
FAB23197MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB23197-SP
Carrier Free
FAB23197G-100UG
Carrier Free
FAB23197V-100UG
Carrier Free
FAB23197U-100UG
Carrier Free
FAB23197T-100UG
Carrier Free
FAB23197S-100UG
Carrier Free
FAB23197R-100UG
Carrier Free
FAB23197N-100UG
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LAG-3 Antibody (874528) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human LAG-3
Leu23-Leu450
Accession # P18627
Specificity
Detects human LAG-3 in direct ELISA.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LAG-3 Antibody (874528) [Unconjugated]

  • CD223 antigen
  • CD223
  • LAG3
  • LAG-3
  • lymphocyte activating 3
  • lymphocyte activation gene 3 protein
  • lymphocyte-activation gene 3
  • Secreted lymphocyte activation gene 3 protein
  • sLAG-3

Background

LAG-3 (Lymphocyte activation gene-3), also known as CD223, is a member of the immunoglobulin superfamily (IgSF). The mature LAG-3 protein is a 496 amino acid (aa) membrane protein with a 421 aa extracellular region which contains four IgSF domains, a 21 aa transmembrane region and a 54 aa cytoplasmic region. LAG-3 and CD4 molecules share < 20% aa sequence homology but have a similar structure (1, 2). Both molecules bind to MHC class II. LAG-3 binds to MHC class II with higher affinity compared to CD4. Both LAG-3 and CD4 genes are located on the distal part of the short arm of chromosome 12.

LAG-3 is an activation-induced molecule, expressed on activated T cells and NK cells, but not on resting T cells. Studies using LAG-3 -/- mice have shown significant delay of T cell apoptosis following antigen stimulation and increased size of memory T cells pool following infection (3, 4). It also has been reported that anti-LAG-3 antibodies up-regulate T cell activation by blocking interaction of LAG-3 and MHC class II. The study has demonstrated that LAG-3 is selectively expressed on activated CD4+CD25+ TReg cells and plays a role in their suppressive activity (5). This evidence indicated, unlike the interaction of CD4 with MHC class II that plays a positive role in T cell activation, LAG-3 binds to MHC class II and negatively regulates T cell activation through LAG-3 signaling. On the other hand, studies have shown that binding of LAG-3 to MHC class II molecules on antigen presenting cells induce maturation of dendritic cells and cytokine secretion by monocytes through MHC class II signal transduction (6). Taken together, LAG-3 may have two major functions, it negatively regulates T cells activation through LAG-3 signaling and stimulates antigen presenting cells which express MHC class II.

  1. Triebel, F. et al. (1990) J. Exp. Med. 171:1393.
  2. Baixeras, E. et al. (1992) J. Exp. Med 176:327.
  3. Workman, C.J. and D.A. Vignali (2003) Eur. J. Immunol. 33:970.
  4. Workman, C.J. et al. (2004) J. Immunol. 172:5450.
  5. Huang, C.T. et al. (2004) Immunity 21:503.
  6. Andreae, S. et al. (2003) Blood 102:2130.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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FAQs for LAG-3 Antibody (MAB23197). (Showing 1 - 2 of 2 FAQ).

  1. Can this LAG-3 antibody be used in ELISA? 
    • We have not validated this particular LAG-3 antibody for ELISA in mouse; however, it has been cited as being used as a detection antibody in a publicatoin (PMID 15946792).
  2. I'm using your LAG-3 antibody in western blot but the observed band is a different size than the predicted band size, do you know what the bands are?
    • There are several alternative splice varients of LAG-3 that could explain the different sized proteins stained by the LAG-3 antibody. It is also believed that there are two secreted forms of LAG-3, one that is about 36 kDa and another at 52 kDa.

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