Human Lactalbumin Alpha ELISA Kit (Colorimetric) Summary
Description |
The stop solution changes color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Human Lactalbumin Alpha in the sample, this Human Lactalbumin Alpha ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of optical density versus Human Lactalbumin Alpha concentration. The concentration of the samples is then determined by comparing the O.D. of the samples to the standard curve. |
Standard Curve Range |
0.78-50 ng/ml |
Sensitivity |
0.38 ng/ml |
Assay Type |
Sandwich ELISA |
Inter-Assay |
%CV < 15 |
Intra-Assay |
%CV < 10 |
Spike Recovery |
80-109% |
Sample Volume |
100 ul |
Kit Type |
ELISA Kit (Colorimetric) |
Gene |
LALBA |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- Balance Solution
- Enzyme Conjugate
- Microtiter Plate
- Standards
- Stop Solution
- Substrate A
- Substrate B
- Wash Solution
|
Alternate Names for Human Lactalbumin Alpha ELISA Kit (Colorimetric)
Background
Alpha-LA is the B protein of lactose synthetase secreted by the mammary epithelial cells. a-LA is a potent Ca(2+)-elevating and apoptosis-inducing agent with broad, yet selective, cytotoxic activity. Multimeric a-LA kills all transformed, embryonic, and lymphoid cells tested but spared mature epithelial elements. It suggests that milk contributes to mucosal immunity not only by furnishing antimicrobial molecules but also by policing the function of lymphocytes and epithelium. a-LA may be helpful in ascertaining the site of origin of metastatic breast tumors
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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