![Immunohistochemistry-Paraffin: beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody [NBP1-88654] - Staining of human placenta, prostate, skeletal muscle and testis using Anti-B4GALT1 antibody NBP1-88654 (A) shows similar protein distribution across tissues to independent antibody NBP1-88655 (B).](http://images.novusbio.com/fullsize/beta-1-4-Galactosyltransferase-1-B4GalT1-Antibody-Immunohistochemistry-Paraffin-NBP1-88654-img0020.jpg "Immunohistochemistry-Paraffin: beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody [NBP1-88654] - Staining of human placenta, prostate, skeletal muscle and testis using Anti-B4GALT1 antibody NBP1-88654 (A) shows similar protein distribution across tissues to independent antibody NBP1-88655 (B).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: LPQLVGVSTPLQGGSNSAAAIGQSSGELRTGGARPPPPLGASSQPRPGGDSSPVVDSGPGPASNLTSVPVPHTTALSLPACPEESPLLVGPMLIEFNMPVDLELVAKQNPNVKM |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | B4GALT1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. |
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| Control Peptide |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody (NBP1-88654)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | B4GALT1 |
![Western Blot: beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody [NBP1-88654] - Analysis in human cell lines HeLa and HEK293 using anti-B4GALT1 antibody. Corresponding B4GALT1 RNA-seq data are presented for the same cell lines. Loading control: anti-HSP90B1.](http://images.novusbio.com/fullsize/beta-1-4-Galactosyltransferase-1-B4GalT1-Antibody-Western-Blot-NBP1-88654-img0013.jpg "Western Blot: beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody [NBP1-88654] - Analysis in human cell lines HeLa and HEK293 using anti-B4GALT1 antibody. Corresponding B4GALT1 RNA-seq data are presented for the same cell lines. Loading control: anti-HSP90B1.")
![Immunocytochemistry/Immunofluorescence: beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody [NBP1-88654] - Staining of human cell line A-431 shows localization to the Golgi apparatus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/beta-1-4-Galactosyltransferase-1-B4GalT1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88654-img0007.jpg "Immunocytochemistry/Immunofluorescence: beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody [NBP1-88654] - Staining of human cell line A-431 shows localization to the Golgi apparatus. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody [NBP1-88654] - Staining of human testis shows strong granular cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/beta-1-4-Galactosyltransferase-1-B4GalT1-Antibody-Immunohistochemistry-Paraffin-NBP1-88654-img0023.jpg "Immunohistochemistry-Paraffin: beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody [NBP1-88654] - Staining of human testis shows strong granular cytoplasmic positivity in Leydig cells.")
![Immunohistochemistry-Paraffin: beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody [NBP1-88654] - Staining of human placenta shows strong granular cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/beta-1-4-Galactosyltransferase-1-B4GalT1-Antibody-Immunohistochemistry-Paraffin-NBP1-88654-img0019.jpg "Immunohistochemistry-Paraffin: beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody [NBP1-88654] - Staining of human placenta shows strong granular cytoplasmic positivity in trophoblastic cells.")
![Immunohistochemistry-Paraffin: beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody [NBP1-88654] - Staining of human prostate shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/beta-1-4-Galactosyltransferase-1-B4GalT1-Antibody-Immunohistochemistry-Paraffin-NBP1-88654-img0021.jpg "Immunohistochemistry-Paraffin: beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody [NBP1-88654] - Staining of human prostate shows strong granular cytoplasmic positivity in glandular cells.")
![Immunohistochemistry-Paraffin: beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody [NBP1-88654] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/beta-1-4-Galactosyltransferase-1-B4GalT1-Antibody-Immunohistochemistry-Paraffin-NBP1-88654-img0022.jpg "Immunohistochemistry-Paraffin: beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody [NBP1-88654] - Staining of human skeletal muscle shows no positivity in myocytes as expected.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")