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Ku70/XRCC6 Antibody (2F7F5) - BSA Free

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Western Blot: Ku70/XRCC6 Antibody (2F7F5) [NBP2-52589] - Analysis using XRCC6 mouse mAb against Hela (1), PC-2 (2), A549 (3), A431 (4), HepG2 (5), K562 (6) cell lysate.
Immunocytochemistry/ Immunofluorescence: Ku70/XRCC6 Antibody (2F7F5) [NBP2-52589] - Analysis of MCF-7 cells using XRCC6 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with ...read more
Immunohistochemistry-Paraffin: Ku70/XRCC6 Antibody (2F7F5) [NBP2-52589] - Analysis of paraffin-embedded endometrial cancer tissues using XRCC6 mouse mAb with DAB staining.
Flow Cytometry: Ku70/XRCC6 Antibody (2F7F5) [NBP2-52589] - Analysis of A431 cells using XRCC6 mouse mAb (green) and negative control (red).
Western Blot: Ku70/XRCC6 Antibody (2F7F5) [NBP2-52589] - Analysis using XRCC6 mAb against HEK293 (1) and XRCC6 (AA: 6-214)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot: Ku70/XRCC6 Antibody (2F7F5) [NBP2-52589] - Analysis using XRCC6 mAb against human XRCC6 (AA: 6-214) recombinant protein. (Expected MW is 49.7 kDa)
Immunohistochemistry-Paraffin: Ku70/XRCC6 Antibody (2F7F5) [NBP2-52589] - Analysis of paraffin-embedded cervical cancer tissues using XRCC6 mouse mAb with DAB staining.
ELISA: Ku70/XRCC6 Antibody (2F7F5) [NBP2-52589] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready
Clone
2F7F5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

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Ku70/XRCC6 Antibody (2F7F5) - BSA Free Summary

Immunogen
Purified recombinant fragment of human Ku70/XRCC6 (AA: 6-214) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
XRCC6
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
Application Notes
This antibody is Cytof ready.
Theoretical MW
69.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Ku70/XRCC6 Antibody (2F7F5) - BSA Free

  • 5'-deoxyribose-5-phosphate lyase Ku70
  • 5'-dRP lyase Ku70
  • 70 kDa subunit of Ku antigen
  • ATP-dependent DNA helicase 2 subunit 1
  • ATP-dependent DNA helicase II 70 kDa subunit
  • CTC box binding factor 75 kDa subunit
  • CTC box-binding factor 75 kDa subunit
  • CTC75
  • CTCBF
  • D22S731
  • EC 3.6.4.-
  • EC 4.2.99.-
  • G22P1
  • G22P1Ku70
  • Ku autoantigen p70 subunit
  • Ku autoantigen, 70kDa
  • Ku70
  • ML8
  • ML8,70 kDa subunit
  • thyroid-lupus autoantigen p70
  • Thyroid-lupus autoantigen
  • TLAA
  • TLAAD22S671
  • X-ray repair complementing defective repair in Chinese hamster cells 6DNA repair protein XRCC6
  • X-ray repair cross-complementing protein 6
  • XRCC6

Background

The Ku autoantigen is a heterodimer of 70kDa (p70) and ~80kDa (p80) proteins. The p70/p80 dimer is important for function of a 460kDa DNAdependent protein kinase that phosphorylates certain transcription factors, including Sp1, Oct-1, p53, and SV40 large T antigen in vitro. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol XRCC6